LOCUS       sequence-94065-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for Chronos-GFP in a floxed/reversed, 
            Cre-dependent manner (Cre turns gene on).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pCAG-FLEX-rc[Chronos-GFP]
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    183..216
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      238..247
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcattggcca caccagccac caccttctga taggcagcct gcacctgagg agtgcggccg
       61 cgaacctctt cgagggacat aacttcgtat aatgtatgct atacgaagtt atgggtcgat
      121 ggtgagatct ggactagagg gtcgatggtg atgcttggat caaaatagga agaatcccaa
      181 tgataacttc gtataaagta tcctatacga agttatacta ggtacctagg ccgcgccgcc
      241 accatggaaa cagccgccac aatgacccac gcctttatct cagccgtgcc tagcgccgaa
      301 gccacaatta gaggcctgct gagcgccgca gcagtggtga caccagcagc agacgctcac
      361 ggagaaacct ctaacgccac aacagccgga gccgatcacg gttgcttccc ccacatcaac
      421 cacggaaccg agctgcagca caagatcgca gtgggactcc agtggttcac cgtgatcgtg
      481 gctatcgtgc agctcatctt ctacggttgg cacagcttca aggccacaac cggctgggag
      541 gaggtctacg tctgcgtgat cgagctcgtc aagtgcttca tcgagctgtt ccacgaggtc
      601 gacagcccag ccacagtgta ccagaccaac ggaggagccg tgatttggct gcggtacagc
      661 atgtggctcc tgacttgccc cgtgatcctg atccacctga gcaacctgac cggactgcac
      721 gaagagtaca gcaagcggac catgaccatc ctggtgaccg acatcggcaa catcgtgtgg
      781 gggatcacag ccgcctttac aaagggcccc ctgaagatcc tgttcttcat gatcggcctg
      841 ttctacggcg tgacttgctt cttccagatc gccaaggtgt atatcgagag ctaccacacc
      901 ctgcccaaag gcgtctgccg gaagatttgc aagatcatgg cctacgtctt cttctgctct
      961 tgg
//
