LOCUS       sequence-94128-e        1308 bp    DNA     linear   UNA 25-MAR-2026
DEFINITION  encodes multiple copies of an epitope tag OLLAS ( not detected by 
            anti GFP ab).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 25, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_mRuby2_smFP OLLAS
FEATURES             Location/Qualifiers
     source          1..1308
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13..54
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             64..105
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             115..156
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             664..705
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             715..756
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             766..807
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             826..867
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             877..918
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             928..969
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1162..1203
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1213..1254
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1264..1305
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
ORIGIN
        1 gccaccatgg ggtccggatt tgccaatgaa cttggaccta gacttatggg taaacagcag
       61 caatcaggct ttgccaacga gttgggccct cggttgatgg gaaagcagca gcagtccggg
      121 ttcgccaacg aactgggccc gaggctcatg gggaagggcc agggaaactc actgatcaag
      181 gaaaacatgc ggatgaaggt ggtgatggag ggctccgtga atggtcacca gttcaagtgc
      241 accggagagg gagagggaaa cccgtacatg ggaactcaga ccatgcgcat taaggtcatc
      301 gaaggaggtc cgctgccgtt cgctttcgat atcctggcca cttcgttcgg aggagggtcg
      361 cgcacgttca tcaagtaccc gaagggaatc ccggacttct ttaagcagtc attcccggaa
      421 ggattcactt gggaacgggt gacccggtat gaagatggag gtgtggtgac tgtcatgcaa
      481 gatacttcgc tggaggatgg gtgcctcgtg taccacgtcc aagtccgcgg agtgaatttc
      541 ccgtccaacg gaccagtgat gcagaaaaag acgaagggtt gggaacctaa tactgaaatg
      601 atgtaccccg cagacggagg gctgaggggc tacacccaca tggcgctgaa ggtcgacgga
      661 ggaagcggat ttgcgaatga gctcggccca cgcctcatgg gcaaacaaca gcaaagcggt
      721 tttgctaacg aactgggtcc ccgcttgatg ggaaagcagc agcagagcgg attcgctaat
      781 gagctgggac cgaggcttat gggaaaggga gggcagcagg gcggttccgg tttcgcgaac
      841 gaattgggac cacgcctgat ggggaaacag caacagtccg gcttcgccaa tgagcttggg
      901 ccacgcctta tggggaagca gcaacaaagc gggttcgcta acgagctggg tcctcggctg
      961 atgggtaaag gaggaggaca tctgtcctgt tcgttcgtga ccacctacag atcaaagaaa
     1021 accgtgggaa acatcaagat gccgggcatt catgccgtcg accaccgcct ggagcggctc
     1081 gaagaatcag acaatgagat gttcgtcgtg caaagagaac atgccgtggc caagttcgcg
     1141 ggactgggag gcggtcaagg aagcggcttt gcaaacgaac tcgggccaag attgatggga
     1201 aagcaacagc agtcgggctt tgcgaacgag ctcggaccaa gactcatggg aaaacaacaa
     1261 caatcgggct tcgcaaacga gcttggaccc agactgatgg gcaaatag
//
