LOCUS       sequence-9428-ex       11089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Liu B, Wang S, Brenner M, Paton JF, Kasparov S
  TITLE     Enhancement of cell-specific transgene expression from a Tet-Off 
            regulatory system using a transcriptional amplification strategy in 
            the rat brain.
  JOURNAL   J Gene Med. 2008 May . 10(5):583-92.
  PUBMED    18324737
REFERENCE   2  (bases 1 to 11089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2008 May . 10(5):583-92."
COMMENT     Sequence Label: pTYF-2xSYN-tTA
FEATURES             Location/Qualifiers
     source          1..11089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..498
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             515..955
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             980..1522
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     primer_bind     complement(1591..1610)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1592..1610)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1884..2005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1921..1940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1975..1994
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2166..2613
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             join(2677..3297,3298..3684)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(2750..2769)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    3917..4141
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4274..4293)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(5314..5331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5485..6073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5565..5584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6244..7035,7036..7104))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6867..6886
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7105..7209)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7277..7295
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7346..7368)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7454..7592
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7507..7526
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8796..8816
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    10437..10670
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggcctaggga taacagggta atgattaatc tgcagagggc cctgcgtatg agtgcaagtg
       61 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca
      121 ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg
      181 ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct
      241 tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca
      301 ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc
      361 ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg
      421 cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg
      481 tcgtgcctga gagcgcaggc tagcctcctg aaagatgaag ctactgtctt ctatcgaaca
      541 agcatgcgat atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc
      601 caagtgtctg aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct
      661 gactagggca catctgacag aagtggaatc aaggctagaa agactggaac agctatttct
      721 actgattttt cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat
      781 aaaagcattg ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga
      841 tagattggct tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc
      901 gacatcatca tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgccgga
      961 attgggatcc atggtgtttc cttctgggca gatctcaaac caggccctgg ccttagcacc
     1021 gtcctctgcc ccagtccttg cccagaccat ggtcccttcc tcagccatgg tacctctggc
     1081 tcagccccca gctcctgccc cagttctaac cccgggtcct ccccagtccc tgtctgcacc
     1141 tgttccaaag agcacccagg ctggggaagg cacgctgtcg gaagccctgc tgcacctgca
     1201 gtttgatgct gatgaagact tgggggcctt gcttggcaac agcacagacc caggagtgtt
     1261 cacagacctg gcatctgtgg acaactcaga gtttcagcag ctcctgaacc agggtgtgtc
     1321 catgtctcac tccacagctg agcccatgct gatggagtac cctgaagcta taactcgcct
     1381 ggtgacaggg tcccagaggc cccctgaccc agctcccaca cccctgggga cctcggggct
     1441 tcccaatggt ctctccggag atgaagactt ctcctccatt gcggacatgg acttctctgc
     1501 tctgctgagt cagatcagct ccagcggcca atctagactg cagctcgacc tcgagggggg
     1561 gcccggtacc aggtaagtgt acccaattcg ccctatagtg agtcgtatta caattcactc
     1621 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggagatccaa tttttaagtg
     1681 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcac agtcccaagg
     1741 ctcatttcag gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat
     1801 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     1861 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     1921 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     1981 tgtccaaact catcaatgta tcttaacgcg ctagccttgc atgcctgcag gtcggagtac
     2041 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
     2101 cctccgagcg gagactctag agggacgcgt atcgatggcg ccagctgcag agggccctgc
     2161 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
     2221 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
     2281 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
     2341 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
     2401 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
     2461 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
     2521 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
     2581 agcggaggag tcgtgtcgtg cctgagagcg cagtcgactc tagagctgca ggcaagcttg
     2641 gtaccgagct cggatccact agtctctaga ggatccatgt ctagattaga taaaagtaaa
     2701 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
     2761 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
     2821 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
     2881 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
     2941 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
     3001 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
     3061 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
     3121 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
     3181 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
     3241 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgcg
     3301 tacagccgcg cgcgtacgaa aaacaattac gggtctacca tcgagggcct gctcgatctc
     3361 ccggacgacg acgcccccga agaggcgggg ctggcggctc cgcgcctgtc ctttctcccc
     3421 gcgggacaca cgcgcagact gtcgacggcc cccccgaccg atgtcagcct gggggacgag
     3481 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat
     3541 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc
     3601 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt
     3661 ggaattgacg agtacggtgg gtagctcgag catgcatcta gatgatgcgg ccgcatcgat
     3721 tagggataac agggtaatgg tacctttaag accaatgact tacaaggcag ctgtagatct
     3781 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc agggtctctc
     3841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3901 cctcaataaa gctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3961 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4021 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4081 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4141 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     4201 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4261 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggctgt
     4321 gcgggagaac ggagttctat tatgactcaa atcagtctcc ccaagcattc ggggatcaga
     4381 gtttttaagg ataacttagt gtgtaggggg ccagtgagtt ggagatgaaa gcgtagggag
     4441 tcgaaggtgt ccttttgcgc cgagtcagtt cctgggtggg ggccacaaga tcggatgagc
     4501 cagtttatca atccgggggt gccagctgat ccatggagtg cagggtctgc aaaatatctc
     4561 aagcactgat tgatcttagg ttttacaata gtgatgttac cccaggaaca atttggggaa
     4621 ggtcagaatc ttgtagcctg tagctgcatg actcctaaac cataatttct tttttgtttt
     4681 ttttttttta tttttgagac agggtctcac tctgtcacct aggctggagt gcagtggtgc
     4741 aatcacagct cactgcagcc tcaacgtcgt aagctcaagc gatcctccca cctcagcctg
     4801 cctggtagct gagactacaa gcgacgcccc agttaatttt tgtatttttg gtagaggcag
     4861 cgttttgccg tgtggccctg gctggtctcg aactcctggg ctcaagtgat ccagcctcag
     4921 cctcccaaag tgctgggaca accggggcca gtcactgcac ctggccctaa accataattt
     4981 ctaatctttt ggctaatttg ttagtcctac aaaggcagtc tagtccccag gcaaaaaggg
     5041 ggtttgtttc gggaaagggc tgttactgtc tttgtttcaa actataaact aagttcctcc
     5101 taaacttagt tcggcctaca cccaggaatg aacaaggaga gcttggaggt tagaagcacg
     5161 atggaattgg ttaggtcaga tctctttcac tgtctgagtt ataattttgc aatggtggtt
     5221 caaagactgc ccgcttctga caccagtcgc tgcattaatg aatcggccaa cgcgcgggga
     5281 gaggcggttt gcgtattggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5341 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5401 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5461 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5521 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5581 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5641 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     5701 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5761 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5821 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5881 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5941 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6001 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6061 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6121 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6181 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6241 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6301 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6361 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6421 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6481 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6541 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6601 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6661 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6721 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6781 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6841 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6901 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6961 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7021 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7081 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7141 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7201 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     7261 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aactgcctcg
     7321 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     7381 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7441 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tactggctta
     7501 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     7561 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     7621 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcgcggg gaggcagaga
     7681 ttgcagtaag ctgagatcgc agcactgcac tccagcctgg gcgacagagt aagactctgt
     7741 ctcaaaaata aaataaataa atcaatcaga tattccaatc ttttccttta tttatttatt
     7801 tattttctat tttggaaaca cagtccttcc ttattccaga attacacata tattctattt
     7861 ttctttatat gctccagttt tttttagacc ttcacctgaa atgtgtgtat acaaaatcta
     7921 ggccagtcca gcagagccta aaggtaaaaa ataaaataat aaaaaataaa taaaatctag
     7981 ctcactcctt cacatcaaaa tggagataca gctgttagca ttaaatacca aataacccat
     8041 cttgtcctca ataattttaa gcgcctctct ccaccacatc taactcctgt caaaggcatg
     8101 tgccccttcc gggcgctctg ctgtgctgcc aaccaactgg catgtggact ctgcagggtc
     8161 cctaactgcc aagccccaca gtgtgccctg aggctgcccc ttccttctag cggctgcccc
     8221 cactcggctt tgctttccct agtttcagtt acttgcgttc agccaaggtc tgaaactagg
     8281 tgcgcacaga gcggtaagac tgcgagagaa agagaccagc tttacagggg gtttatcaca
     8341 gtgcaccctg acagtcgtca gcctcacagg gggtttatca cattgcaccc tgacagtcgt
     8401 cagcctcaca gggggtttat cacagtgcac ccttacaatc attccatttg attcacaatt
     8461 tttttagtct ctactgtgcc taacttgtaa gttaaatttg atcagaggtg tgttcccaga
     8521 ggggaaaaca gtatatacag ggttcagtac tatcgcattt cagggtggcc cgagagctgc
     8581 atccggagta tctagatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     8641 tgaccgccca acgacccccg cccattgacg tcaataatga ctgatgttcc catagtaacg
     8701 ccaataggga ctttccattg acgctaatgg gagtttgttt tggcaccaaa atcaacggga
     8761 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtact
     8821 ctagaaggtc tatataagca gagctcgttt agtgaaccgt ttatactact tatctggtct
     8881 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8941 aagcctcaat aaagcttgcc ttgagtgctc aaagtagtgt gtgcccgtct gttgtgtgac
     9001 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     9061 gcccgaacag ggacttgaaa gcgaaagtaa agccagagga gatctctcga cgcaggactc
     9121 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     9181 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcggt attaagcggg
     9241 ggagaattag ataaatggga aaaaattcgg ttaaggccag ggggaaagaa acaatataaa
     9301 ctaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctt
     9361 ttagagacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     9421 ggatcagaag aacttagatc attatataat acaatagcag tcctctattg tgtgcatcaa
     9481 aggatagatg taaaagacac caaggaagcc ttagataaga tagaggaaga gcaaaacaaa
     9541 agtaagaaaa aggcacagca agcagcagct gacacaggaa acaacagcca ggtcagccaa
     9601 aattacccta tagtgcagaa cctccagggg caaatggtac atcaggccat atcacctaga
     9661 actttaaatg catgggtaaa agtagtagaa gagaaggctt tcagcccaga agtaataccc
     9721 atgttttcag cattatcaga aggagccacc ccacaagatt taaataccat gctaaacaca
     9781 gtggggggac atcaagcagc catgcaaatg ttaaaagaga ccatcaatga ggaagctgca
     9841 gaatgggata gattgcatcc agtgcatgca gggcctattg caccaggcca gatgagagaa
     9901 ccaaggggaa gtgacatagc aggaactact agcaaattaa gagaacaatt tggaaataat
     9961 aaaacaataa tctttaagca atcctcagga ggggacccag aaattgtaac gcacagtttt
    10021 aattgtggag gggaattttt ctactgtaat tcaacacaac tgtttaatag tacttggttt
    10081 aatagtactt ggagtactga agggtcaaat aacactgaag gaagtgacac aatcacactc
    10141 ccatgcagaa taaaacaatt tataaacatg tggcaggaag taggaaaagc aatgtatgcc
    10201 cctcccatca gtggacaaat tagatgttca tcaaatatta ctgggctgct attaacaaga
    10261 gatggtggta ataacaacaa tgggtccgag atcttcagac ctggaggagg cgatatgagg
    10321 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    10381 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    10441 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcacg gtcaatgacg
    10501 ctgacggtac aggccagaca attattgtct gatatagtgc agcagcagaa caatttgctg
    10561 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caaacagctc
    10621 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    10681 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    10741 aaatctctgg aacagatttg gaataacatg acctggatgg agtgggacag agaaattaac
    10801 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    10861 gaacaagaat tattggaatt agataaatgg gcttgcggcc ggaattcaca aatggcagta
    10921 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
    10981 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tactaaaaat
    11041 tcnaaaattt tcgggtttat tacagggaca gcagagatcc actttgggc
//
