LOCUS       sequence-94295-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 604, Emission:
            659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-pBAD
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..754)
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             complement(761..781)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(794..826)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(830..847)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acagccaagc ttcgaattct tacttgtaca gctcgtccat gccattaagt ttgtgcccca
       61 gtttgctagg gaggtcgcag tatctggcca cagccacctc gtgctgctcg acgtaggtct
      121 cattgtcggc ctccttgatt ctttccagtc tgcggtccac aaagtagacg ccgggcatct
      181 tgaggttctt agcgggtttc ttggatctgt atgtggtctt caggttgcag tgcaggtggc
      241 ccccgcccac gagcttcagg gccatgtcgc atctgccttc caggccgccg tcagcggggt
      301 acagggtctc ggtggtggcc tcccagccga gtgttttctt ctgcatcaca gggccgttgg
      361 atgggaagtt cacccctctg agcttgacgt tgtagatcaa gcagccgtcc tggaggctgg
      421 tgtcctgggt aacggtaagc acgcccccgt cttcgtatgt ggtgactctc tcccatgtga
      481 agccctcagg gaaggactgc ttaaagaaat cggggatgcc ctgggtgtgg ttgatgaagg
      541 tcttgctccc gtacataaag caggtggcca ggatgtcgaa tgcgaacggc agggggcctc
      601 cctccaccac cttaatcctc tgggtctggg tgccctcgta gggcttgccc tccccttcgg
      661 tggtgcactt gaagtggtgg ttgttcacgg tgccttccat gtacagcttc atgtgcatgt
      721 tctccttgat cagctcctcg cccttgctca ccatgctcga gccctggaag tacaggttct
      781 cgtacagatc ccgacccatt tgctgtccac cagtcatgct agccatacca tgatgatgat
      841 gatgatgaga accc
//
