LOCUS       sequence-94328-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Par1 with N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Ishii K, Hein L, Kobilka B, Coughlin SR
  TITLE     Kinetics of thrombin receptor cleavage on intact cells. Relation to 
            signaling.
  JOURNAL   J Biol Chem. 1993 May 5. 268(13):9780-6.
  PUBMED    7683662
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1993 May 5. 268(13):9780-6."
COMMENT     Sequence Label: pBJ FLAG-hPar1
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(336..355)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     496..518
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    661..795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(698..717)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     752..771
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
      241 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggt cgaccggtcg
      301 accggtcgac cggtcgacct atggtgcact ctcagtacaa tctgctctga tgccgcatag
      361 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
      421 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
      481 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
      541 gcgcgaggca gccggatcat aatcagccat accacatttg tagaggtttt acttgcttta
      601 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
      661 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
      721 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
      781 tatcatgtct ggatccctcg acgg
//
