LOCUS       sequence-94449-e         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    94..318
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     307..324
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(351..371)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attgggtaca ggcgcgcccc ttactaggcg gccgcgaagt tcctatacct tttgaagaat
       61 aggaacttcg gaataggaac ttctctagga cgtccataga gcccaccgca tccccagcat
      121 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
      181 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
      241 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
      301 ggcaactaga aggcacagtc gaggctgatc agcgagctct agatctatca cactttccgc
      361 tttttcttag gggcggccac gtcctcggtg ccgtcctggg cgtcatcctc gtcgtcgtcg
      421 gtgggaggct tggcccaggt gatgctggct ctcttctcga tgggggtgcc ctggcccctg
      481 ccggtggtgc tcttggtcac cacgatctta tccacgaaca ggcccacgaa caccctcttg
      541 tcgtccacgc tggctctgcc ccaccaggac ttggggccgg tggggtcggc gtcggcatcc
      601 tcggggaa
//
