LOCUS       sequence-94607-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to
            accept gRNA from pUC gRNA Shuttle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: p201N Cas9
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aatcaccagt ctctctctac aaatctatct ctctcgaccg ctagcatgga caagaagtac
       61 tccattgggc tcgatatcgg cacaaacagc gtcggctggg ccgtcattac ggacgagtac
      121 aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag
      181 aacctcattg gcgccctcct gttcgactcc ggggagacgg ccgaagccac gcggctcaaa
      241 agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc
      301 tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt
      361 ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag
      421 gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct tgtagacagt
      481 actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat caaatttcgg
      541 ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga caaactcttt
      601 atccaactgg ttcagactta caatcagctt ttcgaagaga acccgatcaa cgcatccgga
      661 gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct cgaaaacctc
      721 atcgcacagc tccctgggga gaagaagaac ggcctgtttg gtaat
//
