LOCUS       sequence-94754-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DSCR6_pCSdest
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(727..751)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(819..838)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggagcc cgaagcggcg gccggagccc
      181 ggaaggcgcg ggggcgcggc tgtcactgcc ccggggacgc tccctggagg cctccgccac
      241 cgcgcgggcc ggagagcccc gcgccgtggc gaccttggat ccagacacct ggagatgctg
      301 agctgaccag aactggaagg ccgcttgaac ctagggctga ccaacacact tttggatcaa
      361 agggagcctt tgggtttcag catcctgtaa gagtctattt acccatgtca aagcgtcaag
      421 aatacctgcg gagttccggg gagcaagtac tggccagttt cccagtgcaa gccacgattg
      481 acttctacga cgatgagtct actgagtctg cttccgaagc tgaagagcca gaggaaggac
      541 ccccacccct ccatcttctg ccccaggagg tgggaggtcg gcaggaaaat ggcccagggg
      601 gaaagggcag agaccagggc atcaaccaag ggcagcgatc ctcaggaggg ggtgaccact
      661 ggggggaggg tccgctccct caaggtgtct cctcaagggg tggcaagtgc tcctcatcca
      721 aatagaaccc agctttcttg tacaaagtgg tgatcctctc gagcctctag aactatagtg
      781 agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa
      841 ctagaatgca gtg
//
