LOCUS       sequence-94758-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:cstA‡, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1940
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    146..162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(217..303)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cttccttcgg ggcatttacg ccaatacgta cctggttgcc ctttacccct aaaactgtca
       61 cggtgacctc atccccaatc atgagggtct caccaactcg acgagtcaga atcagcattt
      121 tttttcctcc ttacccggga gtatcttgtt atccgctcac aatgtcaatg ttatccgctc
      181 acatttatgg atccgcgcct aggtctaggg cggcggattt gtcctactca ggagagcgtt
      241 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      301 ttgatgcctc tagcacgcgt accatgggcc cttagtgtgc gccttttgcc tgcgccacga
      361 tctcctcgac attttccggc attggttcat acggcgtttc tttcgccgtt ggcttcggat
      421 ctttcaatgc cgcaagtgcc gtcttaatcg agaacagtgc cagaaccacc acgaccacca
      481 taaagaagat ggttaacccg gcatccagac ggttgttgaa caccagttgc gccagctgtg
      541 actcagtata ctgcgacgga atattgccgc tgtcgatcat tgcctggaac ttattagcaa
      601 tggccaggaa gccgactttc gcatccgggc taaacgcttt ctgccagcct gcggtcaggg
      661 tacaaa
//
