LOCUS       sequence-94886-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: GART_pCSdest
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     51..68
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
       61 cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattcg aattcaagga
      121 tcacaagttt gtacaaaaaa gcaggcacca tggcagcccg agtacttata attggcagtg
      181 gaggaaggga acatacgctg gcctggaaac ttgcacagtc tcatcatgtc aaacaagtgt
      241 tggttgcccc aggaaacgca ggcactgcct gctctgaaaa gatttcaaat accgccatct
      301 caatcagtga ccacactgcc cttgctcaat tctgcaaaga gaagaaaatt gaatttgtag
      361 ttgttggacc agaagcacct ctggctgctg ggattgttgg gaacctgagg tctgcaggag
      421 tgcaatgctt tggcccaaca gcagaagcgg ctcagttaga gtccagcaaa aggtttgcca
      481 aagagtttat ggacagacat ggaatcccaa ccgcacaatg gaaggctttc accaaacctg
      541 aagaagcctg cagcttcatt ttgagtgcag acttccctgc tttggttgtg aaggccagtg
      601 gtcttgcagc tggaaaaggg gtgattgttg caaagagcaa agaagaggcc tgcaaagctg
      661 tacaagagat catgcaggag aaagcctttg gggcagctgg agaaacaatt gtcattgaag
      721 aacttcttga cggagaagag gtgtcgtgtc tgtgtttcac tgatggcaag actgtgg
//
