LOCUS       sequence-94908-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: C21orf77_pCSdest
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(535..559)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    603..737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(627..646)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     681..700
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggggcg ccctctcatc tggcaccttc
      181 ctggcctctt tcccaggccc cagttctgtc catgcagctg tgggtgcttc ctgcattgcg
      241 ggtctcacgg ggaggagacg agagtgcccc tggttgagtc aggaaagaat tctatcttca
      301 cgtcgctgcc agcaaatgac cacagcagct tcacgacctc tgcaggaacc tatcttggta
      361 aagaaacggg gcctatgtgg tggccgagcc tcaggtgtgg ccgagcttca ggtgtggccc
      421 ttatgcacag cacagcccaa gcctgtgggc accactcgcc ctgggctgcc tggcacctgg
      481 actccttccc atccttggcc gaggtctgcg tggcccttca gggtcgaatc tagaacccag
      541 ctttcttgta caaagtggtg atcctctcga gcctctagaa ctatagtgag tcgtattacg
      601 tagatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
      661 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
      721 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttca
//
