LOCUS       sequence-94914-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: KCNE1_pCSdest
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(472..496)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    540..674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(564..583)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     618..637
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatgatcct gtctaacacc acagcggtga
      181 cgccctttct gaccaagctg tggcaggaga cagttcagca gggtggcaac atgtcgggcc
      241 tggcccacag gtccccccgc agcggtgacg gcaagctgga ggccctctac gtcctcatgg
      301 tactgggatt cttcggcttc ttcaccctgg gcatcatgct gagctacatc cgctccaaga
      361 agctggagca ctcgaacgac ccattcaacg tctacatcga gtccgatgcc tggcaagaga
      421 aggacaaggc ctatgtccag gcccgggtcc tggagagcta caggtcgtag aacccagctt
      481 tcttgtacaa agtggtgatc ctctcgagcc tctagaacta tagtgagtcg tattacgtag
      541 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      601 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      661 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
      721 tgtgggaggt tttttaattc gcggccgcgg cgccaatgca tt
//
