LOCUS       sequence-94967-e         568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of constitutively active human Akt1 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky RM, Zhao H
  TITLE     Akt isoforms differentially protect against stroke-induced neuronal 
            injury by regulating mTOR activities.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 
            10.1038/jcbfm.2013.132. Epub 2013 Aug 14.
  PUBMED    23942361
REFERENCE   2  (bases 1 to 568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/jcbfm.2013.132"; journalName: "J Cereb Blood Flow Metab"; 
            date: "2013-12"; volume: "33"; issue: "12"; pages: "1875-85"
COMMENT     Sequence Label: pHRIG-Akt1
FEATURES             Location/Qualifiers
     source          1..568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(174..200)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(180..206)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa taacatatag
       61 acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg ggggggggag
      121 ggagaggggc ggatcccggg cccgcggtga gaaccgtttc acagtccagg tcccagactg
      181 gcgtagtcgg ggacgtcgta aggataagcc atggccgtgc tgctggccga gtaggagaac
      241 tgggggaagt ggggcctgcg ctcgctgtcc acacactcca tgctgtcatc ttggtcaggt
      301 ggtgtgatgg tgatcatctg ggccgtgaac tcctcatcaa aatacctggt gtcagtctcc
      361 gacgtgacct ggggcttgaa gggtgggctg agcttcttct cgtacacgtg ctgccacacg
      421 ataccggcaa agaagcgatg ctgcatgatc tccttggcgt cctcggagcc cccgccaagc
      481 ctctgcttgg ggtccttctt gagcagccct gaaagcaagg acttggcctc gggaccaagc
      541 gtgcgcggga agcggatctc ctccatga
//
