LOCUS       sequence-9500-ex        3503 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Martin AC, Kaschube M, Wieschaus EF
  TITLE     Pulsed contractions of an actin-myosin network drive apical 
            constriction.
  JOURNAL   Nature. 2009 Jan 22;457(7228):495-9. doi: 10.1038/nature07522. Epub 
            2008 Nov 23.
  PUBMED    19029882
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07522"; journalName: "Nature"; date: "2009-01-22- 
            22"; volume: "457"; issue: "7228"; pages: "495-9"
COMMENT     Sequence Label: pBS-Squ:mCherry
FEATURES             Location/Qualifiers
     source          1..3503
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1952..1961
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1958..2668
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2103..2121)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2371..2390
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggtaccgatc tggtagcaca actgacaata ggtttttagg attctaatag ccatggtcct
       61 agtctcagta gtattcacat ttgtaatata attttatatt ttataatata atttatagtc
      121 ttaatattta tttattttag gagctccaac aattagcact tttgcacagt aatttttagc
      181 atattctgcg gtattttctg ataaattgaa accgcctcta tgctggtcac acttggcctt
      241 ctcgtcgaac cgaaattata aaagacaaaa gtgcagctgg tcgaaagttg cattaacgaa
      301 acttcgatag tattaaacac tcggcattcg gcagctgtga gtatgaatca aatcaggcgt
      361 aatcaagcaa tcatattcga taaaataaac gaactgaact cccaaagggc gctgccctat
      421 gtgagtgtgc gtgtgtgtta gtgtatgcgt atgagtcagc gtcggcagcg cgcaaatgaa
      481 aacaattgtt tttgtgctat aattccattt aaatgtccat tcgaaagtaa taaatcaacg
      541 aagtcgcaaa catttggccc aacttgagtt ctcgagtgaa tttgggtgct tcaactgcgg
      601 tttttttcgg gccttttaag gctttaattg aaatgcgtgt gccagtgtgt gtgagaatgt
      661 tttcaatgag ccaaatacgt gtgcgatgtt gtttttgtta accgcagttc aacagcattg
      721 ataaactcgc atacacatgc acgcactggc gcagagcgag agcgaaactc ccatacgtgt
      781 cattttcgtt tgcgcgagtg actcagagag ctgcgcatgt gtgcgtgcat gagtcgtact
      841 cacagtgggc gcccgataag gtggacttgt aactgtagcg gaagcgttga gccatcaagc
      901 tgcctttgca ttatcttttc tggcctaaaa ctatattaaa actatttgtt tataaatata
      961 atttgtagtt cgcccatttt aggcaatggc caaaccttca ttccttaaaa aacgttaaag
     1021 agcagcatta agatgttcgt tccgttgtga taagcatatg catggtgtac tcactgtagc
     1081 gagcgtccac tgtatgttga cacagtttgt ttggggagca atgaaaatcg gtttcagcgg
     1141 aattcgctgc aatatgtcta tatatagcca taaatacata cccggccaac gaaatgctaa
     1201 tgtgagtcgt cgtttttcga tttcagatcc acgccgcaca cacatcactc cagcgcgaag
     1261 cactagtgcc accagctcca taggccggct ccatttagct ccattaaccg catcagaacc
     1321 accaatccaa cagcaaccat gtcatcccgt aagaccgccg gacgtcgcgc caccaccaag
     1381 aagcgcgccc aacgcgccac ctccaatgtg ttcgccatgt tcgatcaggc gcagattgcc
     1441 gagttcaagg aggcattcaa catgatcgac cagaaccgtg atggcttcgt cgagaaggag
     1501 gatctgcacg acatgctggc ctcgctgggc aagaacccaa cggacgacta tctcgatggc
     1561 atgatgaacg aagcgcccgg acccatcaac ttcaccatgt tcctcacgct cttcggcgag
     1621 cggctgcagg gcaccgatcc cgaggatgtg atcaagaacg ccttcggctg cttcgacgag
     1681 gagaatatgg gcgtcctgcc cgaggatcgt ctgagggagc tgctgaccac catgggtgat
     1741 cggttcacag acgaggatgt atgttgtcaa gttgtttacg atatcaagtc ggagtgtgcc
     1801 cttacaactt cttttccatt tacattcaca ggtggacgag atgtatcggg aggcgcccat
     1861 caagaacggt ctgttcgact acctcgaatt cacgcgcatc cttaagcacg gtgccaagga
     1921 caaggatgag caggtcgacg gtatcgatgt cgccaccatg gtgagcaagg gcgaggagga
     1981 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     2041 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     2101 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     2161 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     2221 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     2281 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     2341 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     2401 ggaggcctcc tccgagagaa tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     2461 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     2521 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     2581 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     2641 cggcggcatg gacgagctgt acaagtaaca ttcgtagaat tgggatcccc agcagtcgat
     2701 tcactagcca gctgcctctc caatcagcct gtccaaacta tccatcatga gtttcgtttt
     2761 gataatttaa tatatacaag tataaatatt tttttttttt ggtttcgcgc cttctgttct
     2821 ttgattcctg ataatcctgt acttatccgt gcatttgaat taaacaaaaa gtagccaaca
     2881 ccagcgattg ttatgtacga cagtgtagtg tgtgagatgt tggtgccaga taacacgatt
     2941 tcccgaaaaa ctcctttcat aagtattaac tcattgaata tacatatata ttacctatat
     3001 acaaatacat acttatacaa gaatgtggca ataaaccttt gtttttctta cactcgagaa
     3061 cgtgcgcacg tccaaccaaa cagatgtctg cagaaatcct tcagccaaaa gcaactgatc
     3121 aaacaataga aactttggaa tatgtacccc gcatggctaa ctaagcatga tcactagcat
     3181 gatgtgataa aagtatgtag cttcaaatgt ggagggaaat tgaaagagat ttcggaaatt
     3241 agtcaaaatt ttcttggaac cttcgacaag ctgactaatg agttcatgtc caaaagactc
     3301 gaacttgtaa actcatatct tatctcacat tatttttgat tcgaagatat ttcagcttct
     3361 gcaaatatat gtttttcaag aatttattta aaaaaaatca attaaataga tggcttaatg
     3421 ttcgaaacac tcaacgttgt atattacaca ctttttggac aactattaat ttaaaagaaa
     3481 acacgaacat ttgaaaatct aga
//
