LOCUS       sequence-95055-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: C21orf77_pCSdest
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(446..470)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(524..542)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(525..542)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctagattc gaccctgaag ggccacgcag acctcggcca aggatgggaa ggagtccagg
      121 tgccaggcag cccagggcga gtggtgccca caggcttggg ctgtgctgtg cataagggcc
      181 acacctgaag ctcggccaca cctgaggctc ggccaccaca taggccccgt ttctttacca
      241 agataggttc ctgcagaggt cgtgaagctg ctgtggtcat ttgctggcag cgacgtgaag
      301 atagaattct ttcctgactc aaccaggggc actctcgtct cctccccgtg agacccgcaa
      361 tgcaggaagc acccacagct gcatggacag aactggggcc tgggaaagag gccaggaagg
      421 tgccagatga gagggcgccc catggtgcct gcttttttgt acaaacttgt gatccttgaa
      481 ttcgaatcga tgggatcctg caaaaagaac aagtagcttg tattctatag tgtcacctaa
      541 atcaagcttg ctccgacgtc cccaggcaga atggcggttc cctaaacgag cattgcttat
      601 atagacctcc cattaggcac gcctaccgcc catttacgtc aatggaacgc ccatttgcgt
      661 cattgcccct ccccattgac gtcaatgggg atgtacttgg cagccatcgc gggccattta
      721 ccgccattga cgtcaatggg agtactgcca atgtaccctg gcgtacttcc aatagtaatg
      781 tacttgccaa gttactatta atagatattg atgtactg
//
