LOCUS       sequence-95219-e        3163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of T7 sigma fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_44
FEATURES             Location/Qualifiers
     source          1..3163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1040..1071
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     rep_origin      complement(1218..1806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1298..1317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1989..2083)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2104..2763)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2678..2697
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2925..2943
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2947..2990)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2993..3014
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_RNA        3072..3121
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 atgagcatcg cggcgaccct ggagaacgat ctggcgcgtc tggaaaacga aaacgctcgt
       61 ctcgaaaaag acatcgcgaa cctggaacgt gacctggcga aactggagcg tgaagaagcg
      121 tacttcggag gttcaggtgg taagaacact ggtgaaatct ctgagaaagt caagctgggc
      181 actaaggcac tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagagt
      241 tcagtcatga cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa
      301 gataccattc agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag
      361 gctgctggat acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg
      421 gttgaagcaa tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat
      481 aagaagactg gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt
      541 ttccctgtgt ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc
      601 ggtcagttcc gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac
      661 aaacaggagt ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt
      721 aagactgtag tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac
      781 tccttcggta cgattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg
      841 gttgacacat atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag
      901 ttgcacgagt ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc
      961 cgtgacatct tagagtcgga cttcgcgttc gcgtaacgct gcaggagtca ctaagggtta
     1021 gttagttaga ttagcagaaa gtcaaaagcc tccgaccgga ggcttttgac taaaacttcc
     1081 cttggggtta tcattggggc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1141 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1621 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     1681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1741 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1801 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1861 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1921 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg
     1981 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     2041 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
     2101 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     2161 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     2221 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     2281 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     2341 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     2401 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     2461 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     2521 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     2581 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     2641 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     2701 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     2761 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     2821 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac
     2881 ttgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2941 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc
     3001 ggccgcttct agagttgaca gctagctcag tcctaggcta taggctagct actagtctat
     3061 ggactatgtt tctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca
     3121 gcctctacaa ataattttgt ttaatcacac aggaaagtac tag
//
