LOCUS       sequence-95369-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: CSTB_pCSdest
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(362..386)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(440..458)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(441..458)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctagaaat aggtcagctc atcatgcttg gctttgttgg tctggtagtt agataaggtc
      121 aagggcttgt tttcatgagg gagagattgg aacactcgca ggtgtacgaa gtcctcgtcg
      181 ccgacgtgca ccttgatgaa gtagtttgtc cccgcgacca cctggctctt gaatgacacg
      241 gccttaaaca cagggaactt cttgttttct ttctcttcaa gctgggacct cacctggtcg
      301 gcgatgtgct gggtctcggc ggtggccggc tgcgtggcgg agggcgcccc gcacatcatg
      361 gtgcctgctt ttttgtacaa acttgtgatc cttgaattcg aatcgatggg atcctgcaaa
      421 aagaacaagt agcttgtatt ctatagtgtc acctaaatca agcttgctcc gacgtcccca
      481 ggcagaatgg cggttcccta aacgagcatt gcttatatag acctcccatt aggcacgcct
      541 accgcccatt tacgtcaatg gaacgcccat ttgcgtcatt gcccctcccc attgacgtca
      601 atggggatgt acttggcagc catcgcgggc catttaccgc cattgacgtc aatgggagta
      661 ctgccaatgt accctggcgt acttccaata gtaatgtact tgccaagtta ctattaatag
      721 atattgatgt actgccaagt gggccattta ccgtcattga cgtcaatagg gggcgtgaga
      781 acggatatga atgggcaatg agccatccca ttgacgtcaa tg
//
