LOCUS       sequence-95384-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: C21orf56_pCSdest
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(626..650)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(704..722)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(705..722)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctaccagg cgaagagggg cttgccgtcc tccttggaga gctcgcacag gcagttgagc
      121 agcagcagcg agtcgcccag gaacttgggg ggcaccacgt cgatgaccag cttgcgcagc
      181 gcggccgggc tgctgtgcag ggggttggcg cgcaggtcgg gccgctgctt caggattccg
      241 taggtgttga tgaggaactc gctgaacgcc gggtgcacgt cgcggctgta gcccagcttc
      301 tccaggcgcg cgctcagggc caggtagcgc tgcgtcagct cgcgcagctt cctctcgtcc
      361 acggagccgt ccagagactt ggtggaggtc tgctcgatct tctcggggat gttggccacc
      421 gtgaagccgt agagccgcgt cacgcccggg aacacgtagg ccaggatgcg gcggtccagc
      481 tggaaggcga tctcgcccac cacgcgcgcg tccttctcgg cgcccgcgaa gctctggatc
      541 tcattgaggt agtagctcct cctggtcctg tccccggtgg gcaggctgct ctccgagaaa
      601 cacaccttct taggccggac catggtgcct gcttttttgt acaaacttgt gatccttgaa
      661 ttcgaatcga tgggatcctg caaaaagaac aagtagcttg tattctatag tgtcacctaa
      721 atcaagcttg ctccgacgtc cccaggcaga atggcggttc cctaaacgag cattgcttat
      781 atagacctcc cattaggcac gcctaccgcc catttacgtc aat
//
