LOCUS       sequence-95444-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN vector with G418 resistance marker for triple Gateway insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p41Neo 3-F GW
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(359..482)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(503..519)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(503..519)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(556..576)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(556..574)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(595..611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(595..611)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(608..630)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    619..635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(643..649,650..667,668..673))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    688..709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga tacagtagaa
       61 attacagaaa ctttatcacg tttagtaagt atagaggctg aaaatccaga tgaagccgaa
      121 cgacttgtaa gagaaaagta taagagttgt gaaattgttc ttgatgcaga tgattttcag
      181 gactatgaca ctagcgtata tgaataggta gatgttttta ttttgtcaca caaaaaagag
      241 gctcgcacct ctttttctta tttcttttta tgatttaata cggcattgag gacaatagcg
      301 agtaggctgg atacgacgat tccgtttgag aagaacattt ggaaggctgt cggtcgacca
      361 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      421 atttaatata ttgatattta tatcatttta cgtttctcgt tcaacttttc tatacaaagt
      481 tgatagcttg gcgtaatcgg gggatccact agttctagag cggccgccac cgcggtggag
      541 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
      601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      721 ctcactg
//
