LOCUS       sequence-95452-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multicopy with G418 resistance marker for triple Gateway insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p42Neo 3-F GW
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(361..484)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(505..521)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(505..521)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(558..578)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(558..576)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(597..613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(597..613)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(610..632)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    621..637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gatacagtag
       61 aaattacaga aactttatca cgtttagtaa gtatagaggc tgaaaatcca gatgaagccg
      121 aacgacttgt aagagaaaag tataagagtt gtgaaattgt tcttgatgca gatgattttc
      181 aggactatga cactagcgta tatgaatagg tagatgtttt tattttgtca cacaaaaaag
      241 aggctcgcac ctctttttct tatttctttt tatgatttaa tacggcattg aggacaatag
      301 cgagtaggct ggatacgacg attccgtttg agaagaacat ttggaaggct gtcggtcgac
      361 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
      421 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt tctatacaaa
      481 gttgatagct tggcgtaatc gggggatcca ctagttctag agcggccgcc accgcggtgg
      541 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
      601 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      661 agcataaagt gt
//
