LOCUS       sequence-95454-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multicopy vector with Hygromycin resistance marker for triple 
            Gateway insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p42Hyg 3-F GW
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(364..487)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(508..524)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(508..524)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(561..581)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(561..579)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(600..616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(600..616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(613..635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    624..640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgatacag
       61 tagaaattac agaaacttta tcacgtttag taagtataga ggctgaaaat ccagatgaag
      121 ccgaacgact tgtaagagaa aagtataaga gttgtgaaat tgttcttgat gcagatgatt
      181 ttcaggacta tgacactagc gtatatgaat aggtagatgt ttttattttg tcacacaaaa
      241 aagaggctcg cacctctttt tcttatttct ttttatgatt taatacggca ttgaggacaa
      301 tagcgagtag gctggatacg acgattccgt ttgagaagaa catttggaag gctgtcggtc
      361 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      421 atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac ttttctatac
      481 aaagttgata gcttggcgta atcgggggat ccactagttc tagagcggcc gccaccgcgg
      541 tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
      601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      661 ggaagcat
//
