LOCUS       sequence-95470-e       11297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the 
            same operon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Armenti ST, Lohmer LL, Sherwood DR, Nance J
  TITLE     Repurposing an endogenous degradation system for rapid and targeted 
            depletion of C. elegans proteins.
  JOURNAL   Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 
            2014 Nov 5.
  PUBMED    25377555
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.115048"; journalName: "Development"; date: "2014-12"; 
            volume: "141"; issue: "23"; pages: "4640-7"
COMMENT     Sequence Label: pSA120
FEATURES             Location/Qualifiers
     source          1..11297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3963..3980)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3963..3979)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5687..5706)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5806..5828
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5866..5884)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5952..6056
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6057..6125,6126..6917)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6275..6294)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7088..7676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7577..7596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9312..9323)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gatcaccaaa aacggaacgt tgagctggac ggaaatagtg gtaaagtgac atgattatag
       61 tttgaagatt tctaatttca caattagagc aaatgttgtt cggtatttat tttcaacggt
      121 atttatacta ttttccacct ttttctagaa cattcgagct gcttgttgca aaaggagggc
      181 gactcacatt cggtacatgg aaaagtagtg tacacaataa agagacccag atacattttc
      241 cgtctgcgtc tctttgcacc caccgggagt attttcaaac gaatgcatct aggaccttct
      301 agaacattct gtaaggctgc agaatgcggg tatataagga aagcgggctc agaggaagcc
      361 aacacgcttt gttctagtgc atctaaaaaa cttcgaaaat gagtgagtgt tccgcgagta
      421 ccagtcagtt atctactttc tgtgtaaatg agataccatg gaagttggtt ccacaggtaa
      481 aaatcttgaa aaccactcag tttcgacaaa acaaaaattt cagacttgcg acgaattcag
      541 cgaatacgag ttcatagatg aaaaatatag gtgaggatct ctagatatat atctattaaa
      601 ttttataacc ttttcaggcg cctttctgac gtagaatcgt ttgtctggaa gtggtcgccg
      661 tgtggaagat atggaattga tcttccgggc ggtcgtgacg tcattaacca acaactacgt
      721 gaccgtatgg ctgtattcga tctacatacg aacaaatgga ccctatacac catggaccgt
      781 ggtgctttcg aaattccacg agtttgcttt ctctactggg cacgaaacga tataatcggc
      841 acaataaccc ttgtagtaag tgtccgatct accataccta atccaaaaaa taaaatttca
      901 gaaagactat gaagcaggac cggatgatga aattccattg aaattcaagc aatcattctt
      961 ctacgtatct cacgaaacac agacattagt gtactctggt tcatgcaagt accgtacgaa
     1021 taaaacaatc gtagcatctt attggtacca tattttcgaa aatcgacgtg gcgaaccgtt
     1081 cattgatcac aataactacc tgtggttggt attctcaaac ggagtggatt ctctgattct
     1141 tcttccattt attccaactt caacgtgttt caaagcgaaa tgtgtatcat ttgatatgaa
     1201 cgatatcgtc agtcaattca ctggtattcg cgtatttctc gatccatttg tatcatctca
     1261 ctacgtgcca tgggtctaca gaaatgacgt taattttttc attaggattg attcgcattc
     1321 atttatcgag gagcaaaacc gggactatgg tctagatccg caaattgata acccggagac
     1381 gaaaccgcac agaggagtat ttcaacttcg tgtgaatttg aaagatgtca tcgaaaaggg
     1441 cgaaaaagat gcgattcatg cagttaatcc tgaaacaata gagacaagat tcaatcgagg
     1501 tgacattgaa ctatggcgaa aaatcagcga tgagctccgt caagaacatc agagcatagc
     1561 acagcatggc aaaatggttg tagtacaacg atggtcacac tttactgttg atatggggct
     1621 cagcaagaaa atgagaaccg taggatatga aaatattatt tgttgatagt ttattaattt
     1681 aatttcagcg tgttgccgat ttggacgccc gtgattactg ttgcagccat aagccacgta
     1741 attcgtcatt ctgctgtatt gacttggaca gcgagcgaat caaggctgtg gatgctctca
     1801 ttcttgggta caaattaatc tatttttttc ctaagtattt ttttttcaga actgacgtgg
     1861 ttcttcccca tcctagtggc tctgtattcc tatttcgata caaggaaaat tatggtaatc
     1921 cctccaccaa atagacgttt tcttcatcgt aatttttcag ggatgagtta ttgcgagctt
     1981 ccattcttcc agccactttc attgaagcta aaatgtattc aagttctcaa ggaatctgtc
     2041 gaaatcgacc caagttacta tagaatcaaa tgctgcctta aaatgcaaga agaacttgaa
     2101 aaagaaactg ccgaaaattt gtgcacgata aatattcaac aataagctgt ctcatcctac
     2161 tttcacctag ttaactgctt gtcttaaaat ctatgcttct ctttagtatc taaaattttc
     2221 ctagaagctt acaagtatat aaatggtctc ttctcaataa aggttgtata tttattcatc
     2281 ttattgaatc tgccatttcc tcgtttttgc gagtttatat accttccaat tttctttcta
     2341 ttgtattttc aacttctaat tttaattcag ggaaactgct tcaacgcatc atggtctcaa
     2401 agggtgaaga agataacatg gcaattatta aagagtttat gcgtttcaag gtgcatatgg
     2461 agggatctgt caatgggcat gagtttgaaa ttgaaggtga aggagaaggc cgaccatatg
     2521 agggaacaca aaccgcaaaa ctaaaggtaa gtttaaacat atatatacta actaaccctg
     2581 attatttaaa ttttcaggta actaaaggcg gaccattacc attcgcctgg gacatcctct
     2641 ctccacagtt catgtatgga agtaaagctt atgttaaaca tccggcagat ataccagatt
     2701 atttgaaact ttcattcccg gagggtttta agtgggaacg cgtaatgaat tttgaagacg
     2761 gaggagttgt tacagtgacg caagactcaa ggtaagttta aacagttcgg tactaactaa
     2821 ccatacatat ttaaattttc agcctccaag atggagaatt tatttataaa gtcaaacttc
     2881 gaggaacgaa tttcccctcg gatggacctg ttatgcagaa gaagactatg ggatgggaag
     2941 cttcaagtga aagaatgtac cctgaagacg gtgctcttaa gggagagatt aaacaacgtc
     3001 ttaaattgaa agatggagga cattacgatg ctgaggtaag tttaaacatg attttactaa
     3061 ctaactaatc tgatttaaat tttcaggtga agacaactta caaagccaaa aaaccagttc
     3121 agctgccagg agcgtacaat gttaatatta aactggatat cacctcccac aacgaggatt
     3181 acactatcgt tgagcaatat gaaagagctg aagggcggca ctcgacaggt ggcatggatg
     3241 aattgtataa gtaggtccaa ttactcttca acatccctac atgctctttc tccctgtgct
     3301 cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt tttttagctt
     3361 cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat
     3421 aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct
     3481 acacaatgtt ctgtgtacac ttcttatgtt ttttttactt ctgataaatt ttttttgaaa
     3541 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
     3601 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
     3661 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
     3721 cctgcttttg ctttttgggg gtttccccta ttgtttgtca agagtttcga ggacggcgtt
     3781 tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt
     3841 gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct
     3901 atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat aactgtttaa
     3961 acactggccg tcgttttaca ctcgagacgt acggtgcgcg cgatgcattc gaagatctgc
     4021 ccactagtga gtcgtattat aagtgcaagt aagatcagtg tttgtttcga tatcagtctg
     4081 tttcgtaacg gtcttctgta taactacaaa aaagaataaa aaaccgtatc acaactgaga
     4141 aaaaagacaa aacttattga acagtgttcc tttcaaaaaa ttattttgta aaaaatcgag
     4201 tttttctcca aaataacgca cgcaatcaaa aacaagaaaa ctaacgtgtt ctcctgggcg
     4261 tggaactcct tatcaagatt accattattg tctgtcagaa ttgatatttc aattatttca
     4321 tatctagaca aacccgggtg tcttgcattt attagtactg tgagtttaca tacaaacgag
     4381 gcttactcag ataacataga gtcacctcta attagcaaaa acccatttct aattagctat
     4441 ttcgaccagt tctaaagtca ccgtaattag aaatggtttt tgtagctttt ctaattagaa
     4501 atgaccccga gataagtgaa atatggcatt ttctgggttt tctagtaaga aatgatcccg
     4561 cacgtagtga ttccgcgtta caagccaaat tcaaattaaa aaagtatatt acacaattca
     4621 agttacatta ttttcaggaa acaatggcga agagcaaagc agagattcaa aaggaggaaa
     4681 gagacaacag atatgaacga agacttcttg aaaagagaaa gagacatgag gtgagtaacc
     4741 atgataataa ataatcaagt aagggctata gatttataaa ttctggagtt aacagtttcg
     4801 aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa taggggaata gcctaaacga
     4861 gtgagaaaat taaatgtttg ttattttttc aaaaatgtct gcagggccgt tatcctttca
     4921 aatgcactct atcaaacata gatcatatcc atcatatctt atccagaatt tatatcatat
     4981 ccaagattga gagcgtttca aggaaaatct caaacaacca tacaattttg tagatcgaca
     5041 acaaggcctc cacttcttca tcgatggcct ctgcagcacc tgcaccacca agtttgtatt
     5101 tttcatatta atataaagtg agccacgcat agtcgttttt aggatttttg ccagatcgat
     5161 gtcgcgcaaa cagactgttt ttttttcttt gaaagccgtg cgtgacatgg gccacggatt
     5221 gtaacgccgc gctggatgga taaaaattta ttcagttttt tagcctttac aaaatatggt
     5281 tcgactactt tctttatttt tacattttta taggtgctca ttgagctgag atattttggg
     5341 tataatcgga aaatcgctga ttttggtcat ttttctgcac taaagattac acaaaaagtt
     5401 cagtatttag attcattgtt gaaaattggg gatgatctac agatacatat cgccgtgttc
     5461 aaaaacacac gtgctgtgac gtcgtgactg gggaaaaccc tggcgttacc caacttaatc
     5521 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     5581 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     5641 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     5701 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5761 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5821 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5881 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5941 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6001 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6061 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6121 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6181 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6241 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6301 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6361 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6421 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6481 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6541 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6601 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6661 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6721 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6781 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6841 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6901 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6961 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7021 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7081 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7141 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7201 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7261 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7321 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7381 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7441 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7501 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7561 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7621 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7681 ccagcaacgc ggccttttta cggttcctgg tcttttgctg gccttttgct cacatgttct
     7741 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7801 ccagcggata acaatttcac agggccctgt cggattatgg gctcttcttt tatcctctac
     7861 tttctttcta gaaaatgcaa atgttgagtc agaaattgct gaatggagta caactgcaat
     7921 gggtttagct tatgtttctg gaacaattac tggtggaatt gtgattgaaa agtaaggtta
     7981 tatttcattt caataaatgt taccacagta taacaaaaac aagttacatt gttcagagta
     8041 tgtctgaatg tattattatt attgtatttg tgcatcagta ctgtagttgt actgaagaag
     8101 ggtttaaaat aatctcaacg tttttaagac gagttagtga gaatacggtc gcagttagga
     8161 tgtttggaca tagttttatt agataatcgt gaactaactc actataaaaa cccgctaact
     8221 cattgaataa gatagtttta aacgttcaaa atttgaagac aaaattgtat gaataagttt
     8281 acagagttgg tcgacgcaag cttctccttc tcttcacatt tctcaataat ttagctctaa
     8341 ttgcattcgt attcttcgca aaaatacgta ttctgattga cccgatgaaa tacggatgcc
     8401 tagttgcatt gatcatctac ggttatactt atgggtgagg tttttcaaat atattttgat
     8461 ttaatcgaat aatgaaattt cagaactgga gttggtccaa tttcgtggtt tatatcctct
     8521 gaactggtac ctcaaaaaca tcgtagtatt gctcaatctg tggcatattc aatgaatact
     8581 gtaatggtgg ttatcagtac atttacagta ttacctctat attccgttat tggtagttat
     8641 gctttcctta ttctatattc aattccttca tttataagta tgcttatatt atttcgctac
     8701 ttaccggaaa ccaaaggacg agagattcat gatattgtga atgaacttaa aaaaaaatga
     8761 aaacctttga aaacgttcat acggccgaaa ttttacttga tcgagccaat tgaacttttg
     8821 ttaagactta cagaaaagtg agagattttg ttacaaaaaa gtgagaaaaa agcgaaaatg
     8881 aacaggaaaa agaagaaaaa tacacccgaa gtatcatata aaaattaaaa agattgtttg
     8941 acctggcgga acttttttgc aaaaactctg aatatccatg gcacatgctt cgtgcaaaac
     9001 aggatccaaa tgaatatcaa caagagattc ttgaagacga cgagccactt gctcagagca
     9061 ttgcttatct cgaatgagac ccttgttaaa atcatttttc aaacattata tgacattgtc
     9121 gaaatgtcct cctgattcca tgatggtagc aaactcactt cgtctttgag ccaatttatc
     9181 caagtccttg taaaaagtat tcgaaaattg ttaacggata aaatgtttat tataatatca
     9241 aaaacaattt gtcagttgac cactttttga tatatttgac agaaaccggg atgaattggc
     9301 tcaaagttag ggcgcccttc tattacaggg tttctgataa caaaccggtt attaactccc
     9361 aacaagggat gatttcaatt catcatgctc aaattgaccc aaattaagtt acatgacaaa
     9421 ttcatcgccc ttttcaactc ttcttggctc atcatctgtt attctgttcc tattctgtct
     9481 gcaccctata cccctttgca tactctctcg tcattccttt cttggatagt gtcttcttct
     9541 ccccagctct gctacttcta tgacttgcgc ccgctgcttt tccgctcgtt cctctcctct
     9601 ctgacgtctt cttctgcttc ttttctcctt cctttttccc ttctcaatct tcctcttttt
     9661 cccatttctg tcaatcattc gaagaagaag aagaagaaga ccctcattca tttcattttt
     9721 ttttctgtcg gtgtgtgctg ctcggttaag agtgagctct ctattccacg tcttcttctt
     9781 tttcttcttc gattcgaatc aatcactcca ccaaaacgca ttcgtttttg ggattcaccc
     9841 cgcggttcgc aataggtttc tttttcaaat tattagcgtt ataaatagaa aaatggtgga
     9901 gtttcaataa aaatgataat ttacaaagtg tattttgatt acatgtactc aaaaaggttg
     9961 aaatttttca taccagtttt ccggaaatcc atctgatatc attatcgtat ttttcttttt
    10021 aaaaatgttt tcaaaaaaaa acaaaatata gctggttatt tggcaccctc taattaccat
    10081 tttcctgtca caccacactc ttttccttct tccacttctt tccgttttca gccgcttcca
    10141 accaaaccga tatgaaagcc gagcaacaac gatcgattcc acccggctcg gcgaccttcc
    10201 cgtcgcaggt gagactcaga aaactagaga aaccgctcaa ctaactcttg atatccgatt
    10261 tcattctttt ctcttttctt tttgttgaac ttttccatat ttccagatgc cacggccacc
    10321 accaagcacc gaacaaggaa tcacaacgga atcggagctt gcgaagaaag ctcaaatcac
    10381 tccgaacgac gttttagcac ttccgggaat cactcaaggt atcttgctcc ctttggattt
    10441 cagtttaaaa cataaattta ggattcttat gctccccatc tgcgaacatc tataacatcg
    10501 agttcaccaa gttccaaatc cgtgatctgg acactgagca agtgcttttc gagatcgcca
    10561 aaccggagaa cgatcaggag aatgatgagt cgccacagga gtcggcaaga tacgtgcgtt
    10621 atagatttgc tccaaacttt ttgaaactca aaacggtcgg agcaactgtg gaattcaaag
    10681 taggagacat cccaatccat catttccgaa tgatcgaacg tcacttcttc aaagatcgcc
    10741 ttctgaagtg ttttgacttt gaattcggat tctgtattcc gaattcacga aacaactgtg
    10801 aacatatcta tgagttccct caactctctc aacaactcag tgagtcatta ttctaaaaag
    10861 tacaattcaa aagactaatc tctttttcag tggacgacat gatcaacaat ccaaacgaga
    10921 ctcgttctga cagcttctat ttcgtcgata acaaactcgt catgcacaac aaagccgact
    10981 actcatatga tgcataaata tttaatacaa aaatgttctg gataattatt ctgtcgaata
    11041 gaaaaaaaac tccaaatgtg attaaattcc aataattcct gtctagtttg ttccttcccc
    11101 ttccccttct catgttcaat gcattcctaa gcttttcagt tccccccttg tttctatatt
    11161 ttttcgctgt cctgtcacac tcgctaaaaa cactaatcac acggaaatct gttttcaata
    11221 aaaactccaa ctttaactca ttttcaattt caactgaaag attttttcat tagagaatgt
    11281 ctagaactag gccgttt
//
