LOCUS       sequence-9558-ex       10031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Brambrink T, Foreman R, Welstead GG, Lengner CJ, Wernig M, Suh H, 
            Jaenisch R
  TITLE     Sequential expression of pluripotency markers during direct 
            reprogramming of mouse somatic cells.
  JOURNAL   Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
  PUBMED    18371436
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Feb 7. 2(2):151-9."
COMMENT     Sequence Label: TetO-FUW-Klf4
FEATURES             Location/Qualifiers
     source          1..10031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2723
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4492..5080
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4545..4565)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4963..4974)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5083..5099)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5084..5100)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5605..5785
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5811..5828)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5817..6041
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6087..6515
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6174..6193)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6384..6405
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6524..6544)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6529..6858
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6709..6844
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6771..6790
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6906..6953
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6972..7346
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7476..7597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7513..7532)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7567..7586
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7646..7662)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7646..7662)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7659..7681)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7670..7686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7694..7700,7701..7718,7719..7724))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7739..7760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7877..7894)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8048..8636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8128..8147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8807..9598,9599..9667))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9430..9449
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9668..9772)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9847..9866)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
     2821 tcggccatta cggccggttt gggtacccct ctctcttctt cggactccgg aggaccttct
     2881 gggcccccac attaatgagg cagccacctg gcgagtctga catggctgtc agcgacgctc
     2941 tgctcccgtc cttctccacg ttcgcgtccg gcccggcggg aagggagaag acactgcgtc
     3001 cagcaggtgc cccgactaac cgttggcgtg aggaactctc tcacatgaag cgacttcccc
     3061 cacttcccgg ccgcccctac gacctggcgg cgacggtggc cacagacctg gagagtggcg
     3121 gagctggtgc agcttgcagc agtaacaacc cggccctcct agcccggagg gagaccgagg
     3181 agttcaacga cctcctggac ctagacttta tcctttccaa ctcgctaacc caccaggaat
     3241 cggtggccgc caccgtgacc acctcggcgt cagcttcatc ctcgtcttcc ccagcgagca
     3301 gcggccctgc cagcgcgccc tccacctgca gcttcagcta tccgatccgg gccgggggtg
     3361 acccgggcgt ggctgccagc aacacaggtg gagggctcct ctacagccga gaatctgcgc
     3421 cacctcccac ggcccccttc aacctggcgg acatcaatga cgtgagcccc tcgggcggct
     3481 tcgtggctga gctcctgcgg ccggagttgg acccagtata cattccgcca cagcagcctc
     3541 agccgccagg tggcgggctg atgggcaagt ttgtgctgaa ggcgtctctg accacccctg
     3601 gcagcgagta cagcagccct tcggtcatca gtgttagcaa aggaagccca gacggcagcc
     3661 accccgtggt agtggcgccc tacagcggtg gcccgccgcg catgtgcccc aagattaagc
     3721 aagaggcggt cccgtcctgc acggtcagcc ggtccctaga ggcccatttg agcgctggac
     3781 cccagctcag caacggccac cggcccaaca cacacgactt ccccctgggg cggcagctcc
     3841 ccaccaggac tacccctaca ctgagtcccg aggaactgct gaacagcagg gactgtcacc
     3901 ctggcctgcc tcttccccca ggattccatc cccatccggg gcccaactac cctcctttcc
     3961 tgccagacca gatgcagtca caagtcccct ctctccatta tcaagagctc atgccaccgg
     4021 gttcctgcct gccagaggag cccaagccaa agaggggaag aaggtcgtgg ccccggaaaa
     4081 gaacagccac ccacacttgt gactatgcag gctgtggcaa aacctatacc aagagttctc
     4141 atctcaaggc acacctgcga actcacacag gcgagaaacc ttaccactgt gactgggacg
     4201 gctgtgggtg gaaattcgcc cgctccgatg aactgaccag gcactaccgc aaacacacag
     4261 ggcaccggcc ctttcagtgc cagaagtgcg acagggcctt ttccaggtcg gaccaccttg
     4321 ccttacacat gaagaggcac ttttaaatcc cacgtagtgg atgtgaccca cactgccagg
     4381 agagagagtt cagtattttt ttttctaacc tttcacactg tcttcccacg agggccgcct
     4441 cggccctcga aacctcgagg gcgcgccgaa ttcgatatca agcttatcga taatcaacct
     4501 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4561 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4621 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4681 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4741 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4801 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4861 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4921 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4981 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5041 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     5101 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     5161 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     5221 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5281 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     5341 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     5401 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     5461 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     5521 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     5581 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     5641 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5701 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5761 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     5821 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5881 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5941 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6001 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6061 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6121 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6181 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6241 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6301 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6361 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6421 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6481 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     6541 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6601 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6661 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6721 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6781 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6841 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     6901 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     6961 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     7021 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     7081 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     7141 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     7201 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     7261 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     7321 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     7381 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7441 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     7501 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7561 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     7621 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7681 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7741 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7801 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7861 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7921 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7981 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8041 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8101 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8161 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8221 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8281 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8341 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8401 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8461 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8521 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8581 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8641 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8701 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8761 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8821 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8881 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8941 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9001 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9061 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9121 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9181 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9241 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9301 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9361 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9421 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9481 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9541 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9601 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9661 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9781 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     9841 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     9901 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     9961 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
    10021 ggccagatat a
//
