LOCUS       sequence-95619-e         355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  TITLE     Multiplex genome engineering in human cells using all-in-one 
            CRISPR/Cas9 vector system.
  JOURNAL   Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
  PUBMED    24954249
REFERENCE   2  (bases 1 to 355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-23"; volume: "4:5400. doi"; pages: " 
            10.1038/srep05400"
COMMENT     Sequence Label: pX330S-7
FEATURES             Location/Qualifiers
     source          1..355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        64..312
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     64..84
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     235..254
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcaggt ctcagaggca
       61 tgtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
      121 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
      181 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
      241 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
      301 ggacgaaaca ccgggtcttc gagaagacct gttttagagc tagaaatagc aagtt
//
