LOCUS       sequence-95645-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral pBabe puro vector encoding N-terminally FLAG tagged 
            mutant mouse PERK deleted from amino acids M1 to Y502. The remaining
            amino acids are S503 to N1114. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Volmer R, van der Ploeg K, Ron D
  TITLE     Membrane lipid saturation activates endoplasmic reticulum unfolded 
            protein response transducers through their transmembrane domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4628-33. doi: 
            10.1073/pnas.1217611110. Epub 2013 Mar 4.
  PUBMED    23487760
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1217611110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-19- 19"; volume: "110"; issue: "12"; pages: "4628-33"
COMMENT     Sequence Label: Flag_MmPERK_S503_N1114_pBabePuro
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             96..140
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             141..164
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccc agtgtggtgg
       61 tacctcgttt agtgaaccgt cagaattaat tcaccatgtc tgcacttctg atcctagctc
      121 ttgttggagc tgcagttgct gactacaaag acgatgacga caagcttgcg gccgccagca
      181 agaacatccg caagaaggac cctatcctcc tgctgcactg gtggaaggag atattcggga
      241 cgatcctgct ttgcatcgta gccacgacct tcatcgtgcg caggcttttc catcctcagc
      301 cccacaggca gcggaaggag tctgaaactc agtgccagac tgaaagtaaa tacgactccg
      361 tgagtgccga tgtcagtgac aacagctgga atgacatgaa gtactcagga tacgtatccc
      421 gatatctaac agattttgag ccaattcagt gcatgggtcg tggtggcttt ggcgttgtct
      481 ttgaagctaa aaacaaagta gatgactgca attacgctat caagaggatc cggctcccca
      541 acagggagtt ggcacgggag aaggtaatgc gggaagttaa agccttggct aagctggaac
      601 acccaggcat tgtgaggtat ttcaacgcct ggctggaaac cccaccagag aagtggcaag
      661 aagagatgga tgagatctgg ctcaaagacg aaagcacaga ctggccgctc agctccccta
      721 gcccgatgga tgccccatct gttaagatcc gaaggatgga tcctttctct acaaaagagc
      781 agatcgaagt catagctcct tctcctgaaa gaagtcggtc tttctcggtg ggcatttcct
      841 gtggccagac aagctcatcg gagagccagt tctctcccct ggagttctca gggacagact
      901 gcggagacaa cagtgactca gcggacgcag cctacaacct ccaggacagt tgcctgacgg
      961 actgcgagga cgtggaagat ggcaccgtgg acggcaatga cgagggacac tcctttgaac
     1021 tttgtccgtc cga
//
