LOCUS       sequence-95655-e         520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  TITLE     Multiplex genome engineering in human cells using all-in-one 
            CRISPR/Cas9 vector system.
  JOURNAL   Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
  PUBMED    24954249
REFERENCE   2  (bases 1 to 520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-23"; volume: "4:5400. doi"; pages: " 
            10.1038/srep05400"
COMMENT     Sequence Label: pX330S-7
FEATURES             Location/Qualifiers
     source          1..520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(217..292)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(369..388)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatt gacgtcaatg
       61 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgggtc
      121 tcaacctcta gagccatttg tctgcagaat tggcgcacgc gctaaaaacg gactagcctt
      181 attttaactt gctatttcta gctctaaaac aaaaaagcac cgactcggtg ccactttttc
      241 aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa acaggtcttc
      301 tcgaagaccc ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact
      361 ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact
      421 acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag
      481 tcaaattaat tccaattatc tctctaacag ccttgtatcg
//
