LOCUS       sequence-95746-e         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector to express p38 KTR mClover under PGK promoter 
            (With Puromycin Resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pLentiPGK Puro DEST p38KTRClover
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(359..380)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac cggttcgtcg
       61 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc
      121 caccatgcgt aagccagatc tccgcgttct tatcccacct ggcagcaaga acacgatgcc
      181 atcagtgaat caaaggataa ataactccaa gcgctcggcc ggtctcaacg aagacgagtc
      241 gccatctaag aagctggcgt cgccggaggt gtcgtcaagg ctggagcgcc tgaccctcca
      301 gtccagcact agtatggtga gcaagggcga ggagctgttc accggggttg tgcccatcct
      361 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg
      421 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      481 gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca gccgctaccc
      541 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      601 gcgcaccatc tctttcaagg acgacggtac ctacaagac
//
