LOCUS       sequence-95922-e         587 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-P2A-B19G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 587)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-synP-FLEX-EGFP-B19G
FEATURES             Location/Qualifiers
     source          1..587
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..68
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 agggatccgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
       61 ctggacccat ggttcctcag gctctcctgt ttgtacccct tctggttttt ccattgtgtt
      121 ttgggaaatt ccctatttac acgataccag acaagcttgg tccctggagt ccgattgaca
      181 tacatcacct cagctgccca aacaatttgg tagtggagga cgaaggatgc accaacctgt
      241 cagggttctc ctacatggaa cttaaagttg gatacatctt agccataaaa gtgaacgggt
      301 tcacttgcac aggcgttgtg acggaggctg aaacctacac taacttcgtt ggttatgtca
      361 caaccacgtt caaaagaaag catttccgcc caacaccaga tgcatgtaga gccgcgtaca
      421 actggaagat ggccggtgac cccagatatg aagagtctct acacaatccg taccctgact
      481 accgctggct tcgaactgta aaaaccacca aggagtctct cgttatcata tctccaagtg
      541 tggcagattt ggacccatat gacagatccc ttcactcgag ggtcttc
//
