LOCUS       sequence-95923-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: LRRC3_pCSdest
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcgcg cccacccttc
      181 accatgggca ccgtgcgccc acctcgcccc tcgctcctgc tggtctccac ccgggagtct
      241 tgtctcttcc tcctcttctg cctgcacctg ggcgccgcct gcccacagcc ctgccggtgc
      301 cctgaccacg caggggctgt ggctgtcttc tgcagcttgc ggggccttca ggaggtcccc
      361 gaggacatcc cggccaacac cgtgctcctg aagctcgatg ccaacaagat ctcccacctc
      421 ccggacgggg ccttccagca cctgcaccgg ctcagggagc tggatctgtc tcacaacgcc
      481 atcgaggcca tcggctccgc caccttcgcg ggcctggccg ggggcctgcg gctgctggac
      541 ctgtcttaca accgcatcca gaggatcccc aaggacgccc tgggcaaact cagcgccaag
      601 atacgcctgt cccacaaccc cctgcactgc gagtgcgccc tgcaggaggc cctgtgggag
      661 ctgaagctgg accccgactc tgtggacgag atcgcctgcc acacctcagt gcaggaggag
      721 tttgtgggga agcctctggt tcaggctctg gatgcgggtg ccagcctctg cagcgtcccc
      781 cacaggacca cagacgtggc catgctggtc accatgttcg gct
//
