LOCUS       sequence-95938-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: PRMT2_pCSdest
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tcgaattcaa
      121 ggatcacaag tttgtacaaa aaagcaggca ccatggcaac atcaggtgac tgtcccagaa
      181 gtgaatcgca gggagaagag cctgctgagt gcagtgaggc gggtctcctg caggagggag
      241 tacagccaga ggagtttgtg gccatcgcgg actacgctgc caccgatgag acccagctca
      301 gttttttgag aggagaaaaa attcttatcc tgagacaaac cactgcagat tggtggtggg
      361 gtgagcgtgc gggctgctgt gggtacattc cggcaaacca tgtggggaag cacgtggatg
      421 agtacgaccc cgaggacacg tggcaggatg aagagtactt cggcagctat ggaactctga
      481 aactccactt ggagatgttg gcagaccagc cacgaacaac taaataccac agtgtcatcc
      541 tgcagaataa agaatccctg acggataaag tcatcctgga cgtgggctgt gggactggga
      601 tcatcagtct cttctgtgca cactatgcgc ggcctagagc ggtgtacgcg gtggaggcca
      661 gtgagatggc acagcacacg gggcagctgg tcttgcagaa cggctttgct gacatcatca
      721 ccgtgtacca gcagaaggtg gaggatgtgg tgctgcccga gaaggtggac gtgctggtgt
      781 ctgagtggat ggggacctgc ctgctgtttg agttcatgat cgagtccatc ctgtatgccc
      841 gggatgcctg gctgaaggag gacggggtca
//
