LOCUS       sequence-95940-e         457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses splitTVA-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 457)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-FLEX-splitTVA-EGFP
FEATURES             Location/Qualifiers
     source          1..457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..44
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    95..128
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aggttgattc ataacttcgt ataaagtatc ctatacgaag ttattctttg caccattcta
       61 aagaataaca gtgataattt ctgggttaag gcaaataact tcgtataatg tatgctatac
      121 gaagttatta ggtgcgcggc tgctgcccgc gctgctgctg ctgctgctgc ccggtaacgt
      181 gaccggtaac gggtccggta acggttcttt gtcccgttgc ccccccggtc agttccgctg
      241 ctcggagccg cccggtgccc acggggagtg ttacccgcag gactggctgt gcgacggaca
      301 ccccgactgc gacgacgggc gggacgagtg gggctgcggg accagcgcga tccccgcggt
      361 gcccacggac aacggcacag aggctcccac tgcccctgct cctggacgtg ctctgccagc
      421 caggaatcac ggccgcatgt ggatgctgat cactgca
//
