LOCUS       sequence-95972-e         555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: DYRK1A_pCSdest
FEATURES             Location/Qualifiers
     source          1..555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(517..546)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(520..549)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(523..552)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(526..555)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 tacgactcac atatagttct agaggctcga gaggatcacc actttgtaca agaaagctgg
       61 gtcggcgcgc ccacccttct attacgagct agctacagga ctctgttgca cacaaactcc
      121 tgtcatgggg gactcttctc tatcagcccc ttgcctcatt gtcagatgtc cttcagtcat
      181 gtaatgtgca ggacctgtat tagcagaaaa ttggtatgtt ggatgtccag ctatgccagt
      241 ctcttggcgg ggattggagt agacggtcaa attaacgtcc atagctccat tctgtccaaa
      301 gtccaaggta ttagcagcca ctgggcgatt ctggtaggcc tgattgcctt ggttaccagt
      361 agaggaggaa gatgtcgagg aagaagtcgt tgaggaagac agggatgtca tggagtggtg
      421 actgtggcca acctccatag aatcttgggt agaggagcta ttcgttggag aattgtagac
      481 ccttggcctg gtccggttac ccaaggcttg ttgtccatgg tggtggtgat ggtggtggtg
      541 gtggtgatgg tgatg
//
