LOCUS       sequence-96001-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human IFITM3 with an N-terminal myc tag in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Chesarino NM, McMichael TM, Hach JC, Yount JS
  TITLE     Phosphorylation of the antiviral protein interferon-inducible 
            transmembrane protein 3 (IFITM3) dually regulates its endocytosis 
            and ubiquitination.
  JOURNAL   J Biol Chem. 2014 Apr 25;289(17):11986-92. doi: 
            10.1074/jbc.M114.557694. Epub 2014 Mar 13.
  PUBMED    24627473
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.557694"; journalName: "J Biol Chem"; date: 
            "2014-04-25- 25"; volume: "289"; issue: "17"; pages: "11986-92"
COMMENT     Sequence Label: pCMV-myc-hIFITM3
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    541..675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(565..584)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     619..638
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(786..802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(786..802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(799..821)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    810..826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctctaaaagc tgcggaattg tacccgcggg cccaccatgg catcaatgca gaagctgatc
       61 tcagaggagg acctgcttat ggccatggag gcccgaattc ccatgaatca cactgtccaa
      121 accttcttct ctcctgtcaa cagtggccag ccccccaact atgagatgct caaggaggag
      181 cacgaggtgg ctgtgctggg ggcgccccac aaccctgctc ccccgacgtc caccgtgatc
      241 cacatccgca gcgagacctc cgtgcccgac catgtcgtct ggtccctgtt caacaccctc
      301 ttcatgaacc cctgctgcct gggcttcata gcattcgcct actccgtgaa gtctagggac
      361 aggaagatgg ttggcgacgt gaccggggcc caggcctatg cctccaccgc caagtgcctg
      421 aacatctggg ccctgattct gggcatcctc atgaccattc tgctcatcgt catcccagtg
      481 ctgatcttcc aggcctatgg ataggtcgac cgagatctct cgaggtaccg cggccgcggg
      541 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      601 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      661 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
      721 gtgtgggagg ttttttcgga tcctctagag tcgatctgca ggcatgctag cttggcgtaa
      781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acaca
//
