LOCUS       sequence-96026-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    53..105
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      609..618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(645..848)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(674..694)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gatgatggcc atgttattct cctcgccctt gctcaccatg gtggcgaccg gtggatcccg
       61 ggcccgcggt accgtcgact gcagaattcg aagcttgagc tcgagagtgc tcccgccact
      121 gccctccgag ggcaccgcgg ccggactggc aggcgccgcc ggggtgcggg gggcagcgtt
      181 tcgcgcgcgg gcgcgggcca cgccgctgca ggacaccacg ccgtggcgcc ggcggaagcg
      241 gtccagccag ccgttggagg cggtgaagtc gtccatgccc agctcctcgg ctatgcgcag
      301 cgccttctcc ttgaggatga tgcccttgac cggcaggccg gcggcgcgga tctgctggaa
      361 ccaggcgatg agcaagccct cgagcttgtc gtagggagac agcttgttgg tcttgcggca
      421 ggtggaggcc accccgtact tgcgctccga cgccaggatg gcgcgcttgt tcttcaggat
      481 cgtgctcagc gtggacggcg ggatgttgaa gcgccgcgcg atctcgccct tgcgtaggtc
      541 cgggttctcc tccacctcct ggatgatccg cgacttctcc cggaacgtca gctgccgccg
      601 cttggggccc atggtggcta gcggatctga cggttcacta aaccagctct gcttatatag
      661 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
      721 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
      781 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
      841 cgcatcacca tggtaatagc gatgactaat acgt
//
