LOCUS       sequence-96094-e         536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Origin Of Replication: p15a, Selection Markers: Amp, Promoters: 
            PLacUV5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Juminaga D, Baidoo EE, Redding-Johanson AM, Batth TS, Burd H, 
            Mukhopadhyay A, Petzold CJ, Keasling JD
  TITLE     Modular engineering of L-tyrosine production in Escherichia coli.
  JOURNAL   Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 
            10.1128/AEM.06017-11. Epub 2011 Oct 21.
  PUBMED    22020510
REFERENCE   2  (bases 1 to 536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.06017-11"; journalName: "Appl Environ Microbiol"; date:
            "2012-01"; volume: "78"; issue: "1"; pages: "89-98"
COMMENT     Sequence Label: pY3
FEATURES             Location/Qualifiers
     source          1..536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..77
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(105..132)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(148..219)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta
       61 gcgagtcagt gagcgaggaa gcggaatata tccctaggta taaacgcaga aaggcccacc
      121 cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac agataaaacg
      181 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggaga tccttactcg
      241 agtttggatc cttaacaatt gatcgtctgt gccagggcgc tgcgaatttc agaaatcacc
      301 tggctgggtt cgtttgttgc gtcgatgata atatgcgcaa cttcgcgata tagcgcatcg
      361 cgttcttcca gcacttcctg aacttcttcg ctcagcggtt ttcccgttaa ggttggccgt
      421 aaatcttctt ccggtgcagc ttgcagtcgg ttaaccagga ctgatactgg cgcacacaaa
      481 taaaccacga tcccgttatt ttgcatgaag tgacgattaa attccgtcag aataat
//
