LOCUS       sequence-96113-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY cloning compatible conditional shRNA expression vector; TetO
            flanked H1 RNA gene promoter; Kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pENTR-THT
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     149..165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     149..165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     170..189
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        170..188
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    206..305
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    join(358..376,377..399,400..418,419..441,442..460,461..483,
                     484..502,503..525,526..544,545..567,568..586,587..609,
                     610..628)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
       61 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
      121 gagattttga gacacgggcc agagctgcca ggaaacagct atgaccatgt aatacgactc
      181 actatagggg atatcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg
      241 ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagc
      301 tgggtcggcg cgcccaccct tggatcgatc cggccttgcc ggcctcgagt ttaccactcc
      361 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      421 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      481 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      541 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      601 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggctgc
      661 aggaattcga acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta
      721 ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag
      781 tggcgccctg caatatttgc atgtcgctat gtgttct
//
