LOCUS       sequence-96161-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) 
            cDNA allele F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   McBride CS, Baier F, Omondi AB, Spitzer SA, Lutomiah J, Sang R, 
            Ignell R, Vosshall LB
  TITLE     Evolution of mosquito preference for humans linked to an odorant 
            receptor.
  JOURNAL   Nature. 2014 Nov 13;515(7526):222-7. doi: 10.1038/nature13964.
  PUBMED    25391959
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2014-11-13- 13"; volume: "515"; issue: "7526"; 
            pages: "222-7"
COMMENT     Sequence Label: pUASTattb AaegOr4-F
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    140..173
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    206..300
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        319..557
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     504..525
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt gttgtttata
       61 tatatcaaaa ataacaacta taataataag aatacattta atttagaaaa tgcttggatt
      121 tcactggaac taggctagca taacttcgta taatgtatgc tatacgaagt tatgctagcg
      181 gatccaagct tgcatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc
      241 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
      301 cggagactct agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt
      361 caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc
      421 agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta
      481 accagcaacc aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat
      541 acaagaagag aactctgaat agggaattgg gaattcatga ctcaatccat tgagtttgac
      601 caaacctttc gcttcatagc caaggtcttg caaatgatcg gttacccaag ctgtttggct
      661 ccgtacccaa cgaccttcac cagtcgactt aagt
//
