LOCUS       sequence-96166-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter containing four copies of the Smad binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Zawel L, Dai JL, Buckhaults P, Zhou S, Kinzler KW, Vogelstein B, 
            Kern SE
  TITLE     Human Smad3 and Smad4 are sequence-specific transcription 
            activators.
  JOURNAL   Mol Cell. 1998 Mar . 1(4):611-7.
  PUBMED    9660945
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Mar . 1(4):611-7."
COMMENT     Sequence Label: SBE4-Luc
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        128..324
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      175..310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      354..363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(426..444)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggtgccagaa catttctcta tcgataggta ctaagtctag acggcagtct agacgtacta
       61 agtctagacg gcagtctaga cgtaccgagc tcttacgcgt gctagcccgg gctcgagatc
      121 tgcgatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      181 cccctaactc cgcccagttc cgcccattct ccgccccatc gctgactaat tttttttatt
      241 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
      301 tttggaggcc taggcttttg caaaaagctt ggcattccgg tactgttggt aaagccacca
      361 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
      421 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
      481 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
      541 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
      601 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
      661 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt
      721 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaa
//
