LOCUS       sequence-96231-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Magnetic cell sorting of ES cell-derived astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Bryson JB, Machado CB, Crossley M, Stevenson D, Bros-Facer V, 
            Burrone J, Greensmith L, Lieberam I
  TITLE     Optical control of muscle function by transplantation of stem 
            cell-derived motor neurons in mice.
  JOURNAL   Science. 2014 Apr 4;344(6179):94-7. doi: 10.1126/science.1248523.
  PUBMED    24700859
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-04-4- 4"; volume: "344"; issue: 
            "6179"; pages: "94-7"
COMMENT     Sequence Label: tol2-GFAP::CD14
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     63..79
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(113..124,125..312)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
       61 gctctagaac tagtggatct gctgggcttg ctgaaggtag ggggtcaaga accagaggtg
      121 taaagtactt gagtaatttt acttgattac tgtacttaag tattattttt ggggattttt
      181 actttacttg agtacaatta aaaatcaata cttttacttt tacttaatta cattttttta
      241 gaaaaaaaag tactttttac tccttacaat tttatttaca gtcaaaaagt acttattttt
      301 tggagatcac ttcattctat tttcccttgc tattaccaaa ccaattgaat tgcgctgatg
      361 cccagtttaa tttaaatgtt atttattctg cctatgaaaa tcgttttcac attatatgaa
      421 attggtcaga catgttcatt ggtcctttgg aagtgacgtc atgtcacatc tattaccaca
      481 atgcacagca ccttgacctg gaaattaggg aaattataac agtcaatcag tggaagaaaa
      541 tggaggaagt atgtgattca tcagcagctg cgagcagcac agtccaaaat cagccacagg
      601 atcaagagca cccgtggccg tatcttcgca gaagcttggg ctgcaggtcg agggatctcc
      661 ataagagaag agg
//
