LOCUS       sequence-96343-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak 
            mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre 
            attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK331
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      520..529
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acattgtaat cgcaagcaga gtgttgagag aacggctaac cggatcacca tgtgcagcat
       61 tcattggaga tgacaatatc gtgaaaggag tcaaatcgga caaattaatg gcagacaggt
      121 gcgccacctg gttgaatatg gaagtcaaga ttatagatgc tgtggtgggc gagaaagcgc
      181 cttatttctg tggagggttt attttgtgtg actccgtgac cggcacagcg tgccgtgtgg
      241 cagaccccct aaaaaggctg tttaagcttg gcaaacctct ggcagcagac gatgaacatg
      301 atgatgacag gagaagggca ttgcatgaag agtcaacacg ctggaaccga gtgggtattc
      361 tttcagagct gtgcaaggca gtagaatcaa ggtatgaaac cgtaggaact tccatcatag
      421 ttatggccat gactactcta gctagcagtg ttaaatcatt cagctacctg agaggggccc
      481 ctataactct ctacggctaa cctgaatgga ctacgacatg ccaccatggt gtctaagggc
      541 gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac
      601 caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
      661 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc
      721 ttcatgtacg gcagcaaaac cttcatcaac cacacccagg gcatccccga cttctttaag
      781 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cggg
//
