LOCUS       sequence-96364-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI 
            mutated)-P2A-EBFP2-4xFF5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK332
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..441
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             55..441
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
ORIGIN
        1 ccggatctgg ggcgtgatcc gaaaggtgac ccggatccac cggtcgccac catggcttct
       61 aactttactc agttcgttct cgtcgacaat ggcggaactg gcgacgtgac tgtcgcccca
      121 agcaacttcg ctaacggggt cgctgaatgg atcagctcta actcgcgttc acaggcttac
      181 aaagtaacct gtagcgttcg tcagtcctct gcgcagaagc gcaaatacac catcaaagtc
      241 gaggtgccta aagtggcaac ccagactgtt ggtggtgtag agcttcctgt agccgcatgg
      301 cgttcgtact taaatatgga actaaccatt ccaattttcg ccacgaattc cgactgcgag
      361 cttattgtta aggcaatgca aggtctccta aaagatggaa acccgattcc ctcggccatc
      421 gcagcaaact ccggcatcta ctcgatcgcc atgccagcgg caactgtaga tcatagccaa
      481 agaatttgtg aagtttgggc ttgcaacttg gatgaagaga tgaagaaaat tcgtcaagtt
      541 atccgaaaat ataattacgt tgctatggac accgagtttc caggtgtggt tgcaagaccc
      601 attggagaat tcaggagcaa tgctgactat caataccaac tattgcggtg taatgtagac
      661 ttgttaaaga taattcagct aggactgaca tttatgaatg agcaaggaga ataccctcca
      721 ggaacttcaa cttggcagtt taatt
//
