LOCUS       sequence-96377-e       10670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse NAIP1 expressed under the constitutive, moderate-level 
            retroviral LTR promoter, expresses in mammalian cells; IRES-GFP 
            reporter downstream of NAIP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Kofoed EM, Vance RE
  TITLE     Innate immune recognition of bacterial ligands by NAIPs determines 
            inflammasome specificity.
  JOURNAL   Nature. 2011 Aug 28;477(7366):592-5. doi: 10.1038/nature10394.
  PUBMED    21874021
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-08-28- 28"; volume: "477"; issue: "7366"; 
            pages: "592-5"
COMMENT     Sequence Label: mscv2.2-NAIP1
FEATURES             Location/Qualifiers
     source          1..10670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     57..79
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     95..111
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4405..4971
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4421..4973
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4565..4582)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4792..4811
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4985..4987,4988..4990,4991..5704)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5030..5051)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5291..5310)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5638..5659
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5757..6271
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6440..6456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6440..6456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6453..6475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6464..6480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6488..6494,6495..6512,6513..6518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6533..6554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6671..6688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6842..7430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6922..6941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7601..8392,8393..8461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8224..8243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8462..8566)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8634..8652
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8690..8712)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8812..8831
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9025..9047
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9175..9194
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             9394..9910
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    9974..10315
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             join(10382..10670,1..127)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgcccgt ctctccccct
       61 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
      121 aggcgccgga attagatccg ccaccatggc tgagcatggg gagtcctccg aggatcggat
      181 ttctgagatt gattatgaat ttctccctga gctctctgct cttctcgggg tggatgcagt
      241 tcagctggca aagagccaag aagaagaaga gcataaagaa cggatgaaaa tgaagaaagg
      301 ttttaactca cagatgcgca gtgaagccaa acgactaaag acctttgaga cctatgacac
      361 gttcagatca tggacgccac aggagatggc agctgctggg ttttaccaca caggggtgaa
      421 acttggggtt cagtgctttt gctgtagctt gatcctcttt ggcaatagcc tcaggaagct
      481 tcccatagag agacacaaga aattacgtcc agaatgtgag ttccttcagg gaaaagatgt
      541 tggtaacatt ggcaagtatg acatccgggt gaagagtcca gagaagatgc tgagaggtgg
      601 caaagccagg taccatgaag aggaggccag actggagtcc tttgaggact ggccatttta
      661 tgcccatggg acatcaccac gtgtactctc agcagctggc tttgtcttta caggtaaaag
      721 ggacactgtg cagtgttttt cctgtggcgg aagcttgggc aactgggaag aaggagatga
      781 cccctggaag gagcatgcca agtggttccc caaatgtgaa tttcttcaaa gtaagaaatc
      841 ttcagaggaa attgcccagt atatccaagg ctatgaggga tttgttcatg taacgggaga
      901 acattttgtg aattcctggg tcagaagaga attacctatg gtatcagctt actgcaacga
      961 cagcgtcttc gctaatgaag aactaaggat ggacacgttt aaggactggc cccacgaatc
     1021 acctgtggct gttgatgctt tggtcagagc tggccttttc tacacaggca aaaagggcat
     1081 tgtccagtgt ttttcctgtg gaggatgtat ggagaagtgc acagaaggtg acgacccaat
     1141 acaagagcac aacaagtttt ttcccaactg tgtatttctc caaaccccga agtcctctgc
     1201 agaagtgatt ccagcccttc agagccactg tgcacttcca gaagccatgg aaaccacaag
     1261 tgaaagcaac cacgatgatc cagcagcagt tcattctaca gtggtgggct tgggtaggag
     1321 tgaagcccag tggtttcaag aggccaggag tctgagtgag cagctaagag acaactacac
     1381 taaagccact tttcgccaca tgaacttgcc agaagtgtgc tccagccttg gcactgacca
     1441 cttgatcggc tgcgatgtgt ccatcatttc aaagcacatc agccagcctg tgcaaggggc
     1501 cctgacgatc cctgaggtct tctccaatct cagctctgtc atgtgtgtgg agggggaaac
     1561 tggcagtgga aagacaacct tcctgaagag gatagctttt ctctgggcat caggatgctg
     1621 ccccttgttg tacaggttcc agctggtctt ctacctctcc cttagttcca tcacaccaga
     1681 ccagggactg gccaacatca tctgtgccca actcctaggg gcaggaggct gcattagtga
     1741 agtgtgtctg agcagcatca tccagcagtt acaacaccag gtgctgttcc tgttggatga
     1801 ctacagtggg ctggcctcac tcccccaagc cctacataca ctgattacaa aaaactactt
     1861 gtcacggacc tgcttattga tcgctgtgca tacaaacagg gtcaggggca tccgctcata
     1921 cctagatacg agtctagaga tcaaagagtt tcccttgtcc aatactgtct atatattaaa
     1981 gaagttcttc tcccataata taaaacgtct actagagttt atggtttatt ttggacagaa
     2041 tgaagattta cagggaattc acaagacccc cctcttcgtg gcagcagtat gtactgactg
     2101 gtttgaaaat ccatctgacc agccctttca ggatatggca cttttcaagt cctacatgca
     2161 atacctgtcc ttaaagcaca aaggtgcagc taagcctctc caagccaccg tgtcctcatg
     2221 tgggcagctg gccttgacag ggcttttctc atcatgcttt gagttcaata gtgatgacct
     2281 ggcagaggca ggagttgatg aagatgaaga gctcaccacc tgcttgatga gcaaattcac
     2341 cgcccagaga ctgaggccag tctaccggtt tttaggtccg ctgttccagg agtttcttgc
     2401 tgccatgaga ctgactgaac ttctgagttc agataggcag gaagaccaag atctgggact
     2461 ttattatttg agacaaatta actcaccctt gaaggctttg accacctaca acaatttttt
     2521 gaagtatgtc tttagccatc cgtcatcaaa ggcagggcca acagttgtat ctcatttgct
     2581 tcacttggtg gatgagacag agttgctgga gaatacctat aaaaatgagg attatgtaaa
     2641 tcaccctcca ggaacttccc gaataatgaa gggacttaag gaactgtggc tgttatctcc
     2701 tgaatattac tcttcatttg tttcagaaca tttattgcgc attgctctaa actttgctta
     2761 tgaaagcaac actgttgctg aatgttctcc atttattttg cagttcctta gagggagaac
     2821 actggctttg aaagtactga atttacaata ctttagggac cacccagaaa gcctgttact
     2881 ggtgaagagt ttagaagtct cgataaatgg aaataaagtg ccaaaagttg tagattattc
     2941 agtcatggaa aaaagttttg aaacattaca gccaccaact atagatcagg actatgcatc
     3001 cgcctttgaa caaatgaagg aacatgaaaa aaatttatct gaaaatgaag agaccataaa
     3061 gagtattaag aacatcttcc ccttacagcc acctaaaata agctctggct attggaaact
     3121 gtcccccaag ccatgcaaga tccctaggct ggaagttgga gtgaccaaca tgggtccagc
     3181 agatcaagca ctgctccagg tcctcatgga agtcttctca gcttcacaga gtattgagtt
     3241 ccgtttatcc gacagcagtg gcttccttga aagcatccgc ccagctctgg agctgagtaa
     3301 ggcctctgtc accaagtgtt ccatgtccag gctggagctc agcagagcag aacaggagct
     3361 gcttctcacc ctgcctgccc tgcagtctct cgaggtctca gagacaaacc agttaccaga
     3421 tcagctcttc cataacttgc acaagttcct gggcctgaaa gaactgtgtg tgagactaga
     3481 tggcaaaccg gatgtgctct cagtccttcc tggagagttc ccaaacctcc ttcacatgga
     3541 gaagttatcc atccgaacct ccatggagtc tgacctctcc aaactagtta aattgattca
     3601 gaactctcca aatctccatg ttttccatct gaaatgtgat ttcctttcga attgtgactc
     3661 tctcatggct gtgcttgctt cctgcaagaa actcagagag attgagtttt ctggacgatg
     3721 ctttgaagcc atgccctttg ttaacatttt gccaaatttt atttctctga agatattaaa
     3781 tcttataagc caacaattcc cagataagga aacatcagaa aagtttgccc aggctctggg
     3841 ttctctcagg aacctggagg aactgctcgt tcccactgga gacgggattc accaagtggc
     3901 caaattgatt gtccggcagt gtctgcagct tccgtgtctc cgagttcttg cctttcacta
     3961 catcttggac aatgacagtg tgattgaaat tgccagggtg gcaaccagtg gaggtttcca
     4021 gaaactcgag aagttagatc tttccatgaa tcacaagatt accgaggaag gatatagaaa
     4081 tttctttcaa gccctggaca acctgccaaa cttgcagaat ctgaacatct gcagacatat
     4141 cccagaatgc attcaagttc aggccaccac tgtcaaggct ctgggtcaat gtgtgtcccg
     4201 actgcccagc ctcactaggc tgcacatgct cagttggctc ctggatgaag aggacatgaa
     4261 agtgattaat gatgtgaagg aaagacaccc ccagtccaaa cgcttgatta tcttctggaa
     4321 atggatagtc ccgttctctc ctgttgtcct ggagtaagcg gccgcgttta aacgtcgacg
     4381 gtatcgataa gcttgatatc gaattccgcc ccccccccta acgttactgg ccgaagccgc
     4441 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     4501 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     4561 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     4621 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     4681 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     4741 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     4801 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     4861 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     4921 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     4981 aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     5041 ggacggcgac gtaaacggcc acaagttcag cgtgtctggc gagggcgagg gcgatgccac
     5101 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     5161 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     5221 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     5281 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     5341 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     5401 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     5461 gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     5521 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     5581 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     5641 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     5701 gtgataagaa ttcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     5761 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     5821 aaatacataa ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa
     5881 tatgggccaa acaggatatc tgtggtaagc agttcctgcc cccggctcag ggccaagaac
     5941 agatggtccc cgatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca
     6001 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     6061 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     6121 actcggcgcg ccagtcctcc gatagactgc gtcgcccggg tacccgtgta tccaataaac
     6181 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     6241 gattgactac ccgtcagcgg gggtctttca tgggtaacag tttcttgaag ttggagaaca
     6301 acattctgag ggtaggagtc gaatattaag taatcctgac tcaattagcc actgttttga
     6361 atccacatac tccaatactc ctgaaatagt tcattatgga cagcgcagaa gagctgggga
     6421 gaattaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6481 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6541 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6601 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6661 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6721 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6781 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6841 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6901 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6961 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7021 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7081 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7141 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7201 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7261 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7321 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7381 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7441 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7501 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7561 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7621 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7681 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7741 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7801 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7861 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7921 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7981 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8041 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8101 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8161 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8221 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8281 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8341 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8401 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8461 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8521 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8581 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     8641 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     8701 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     8761 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     8821 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     8881 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     8941 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     9001 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa
     9061 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     9121 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     9181 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     9241 tccggcgtag aggcgattag tccaatttgt taaagacagg atatcagtgg tccaggctct
     9301 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat agataaaata
     9361 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     9421 caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag
     9481 aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg
     9541 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc
     9601 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     9661 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     9721 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
     9781 tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt tgcatccgaa
     9841 tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc cacctcgggg
     9901 gtctttcatt tggaggttcc accgagattt ggagacccct gcccagggac caccgacccc
     9961 cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt
    10021 ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact agctctgtat
    10081 ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg
    10141 tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt cgatgtggaa
    10201 tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
    10261 tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg
    10321 ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta
    10381 gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg
    10441 gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc
    10501 agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat
    10561 cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc
    10621 ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
//
