LOCUS       sequence-96472-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable 
            for in vitro transcription (T7 promoter) for injecting mRNA into 
            oocytes. This LBR contains N-terminal domain only..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-LBR-EGFP-polyA
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..129
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     369..390
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      432..441
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
       61 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
      121 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
      181 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
      241 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
      301 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
      361 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
      421 gcccttgctc accatggtgg cgaccggtgg atccgggtcc ttctgcctgc actgcagcag
      481 cagcaagagg acgcaggcgg gcaggcccag catgatgagg accgcaccag gtactcctcc
      541 aaactccaag tccttcctct gtggagtggt cacttgaaag gttcccaaag gtgcgggtcc
      601 tttggtgaca aggttttgtt cctcagactc tatttctttg gctttgacct cttctctcct
      661 gggacgaagg ctgtactgtg taactatata tctgtcttct gttgacaaaa tgattctttc
      721 ctgcttgtct ttatacggcg tagcgttctt ctccatgtgt tcgggctccc cgttgtacac
      781 gctgacgctg tttccgaacg gcttcaacac aagcggagtc agctt
//
