LOCUS       sequence-96497-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding JNK KTR mClover flanked by Gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-JNKKTRClover
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(346..367)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 aagcaatgct tttttataat gccaactttg tacaaaaaag caggctccac catgagtaac
      121 cctaagatcc taaaacagag catgaccttg aacctggccg acccggtggg cagtctgaag
      181 ccgcacctcc gcgccaagcg ctcggccggt ctcaacgaag acgagtcgcc atctaagaag
      241 ctggcgtcgc cggaggtgtc gtcaaggctg gagcgcctga ccctccagtc cagcactagt
      301 atggtgagca agggcgagga gctgttcacc ggggttgtgc ccatcctggt cgagctggac
      361 ggcgacgtaa acggccacaa gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac
      421 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      481 ctcgtgacca ccttcggcta cggcgtggcc tgcttcagcc gctaccccga ccacatgaag
      541 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctct
      601 ttcaaggacg acggtaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      661 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      721 aagctggagt acaacttcaa cagccacaac gtctatatca cggccgacaa gcagaagaac
      781 ggcatca
//
