LOCUS       sequence-96519-e        3429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains a human pancreatic islet active enhancer, 
            which can be shuttled into a Gateway destination vector, such as 
            pGL4.23-Gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pENTR D-TOPO-C3_7
FEATURES             Location/Qualifiers
     source          1..3429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1554..1653)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1670..1689)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1671..1689)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1694..1710)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1694..1710)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1812..1831)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1823..2632
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1890..1909)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2779..3367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3268..3287
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccgg aggtcagagt tgcctacaag gagaatgggt
      721 gttccagtct tccaggcaag aaaagatgac agtcataacc aggaggtcac tgcaggagaa
      781 tgggaggagg gctgagaagt accttggaga tggagccaag agaacttggt cctgaagaaa
      841 agagatggtg tcagcataga tgaaatgaaa ggaacccttt caggcatgaa gggaaagcta
      901 accaagagct cactctgtgg aagctaaaca gttaattcgc aagaggaagc tgctaatcaa
      961 atgaacaagt gtgaagtgaa aatgccaacc ccagtgaaga cgcagccaaa gctggcaaag
     1021 gcaaatggag aaaacgcagg cagcatcagt gagctgttcc tggaaatgga gtctaactac
     1081 attaacgcat tcagacagag ggaaacagat ggtcggtgtg atgtcctcag tcacttcaca
     1141 agctggataa ataattgttg ccagggaagg aataataaaa gcaggatttt gccaacatga
     1201 gctgttgccc tggcctttgg ttccgggagt gcttctggag gtgaggaaaa agaaaataaa
     1261 ttgagagtga tggagggaaa tcaattagcc cctcacagat gggcctctga catcctagtg
     1321 actgttattg tcgctaatat gagaggttaa gtaagatggg aagagaaata acatggaggg
     1381 attgtatcca gggcttcctg agataacctt acagcagcag gagcactcaa agtgcttgtg
     1441 gaacagctgt ggaggacagg gcagggacca agccagccaa gccaagctcc cccgagggtg
     1501 tggaaagtca ggttgacagg aatggcctga atccatgaag ggtgggcgcg ccgacccagc
     1561 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     1621 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     1681 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     1741 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     1801 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     1861 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1921 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     1981 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2041 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2101 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     2161 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2221 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2281 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2341 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     2401 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2461 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2521 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2581 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     2641 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
     2701 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     2761 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2821 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2881 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     2941 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3001 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3061 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3121 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     3181 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3241 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3301 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3361 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3421 tcacatgtt
//
