LOCUS       sequence-96550-e         451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High core fragment expression without SynZIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_40
FEATURES             Location/Qualifiers
     source          1..451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        78..112
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
ORIGIN
        1 gggcaagtat tgacatgtcg tcgtaacctg tagaacggag taacctcggt gtgcggttgt
       61 atgcctgctg tggattgttt acggctagct cagtcctagg tactatgcta gctactagag
      121 tcatttatga aagtactaga tgaacacgat taacatcgct aagaacgact tctctgacat
      181 cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg
      241 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat
      301 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat
      361 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc
      421 taagcgcggc aagcgcccga cagccttcca g
//
