LOCUS       sequence-96568-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GATEWAY-compatible lentiviral conditional RNAi delivery vector; 
            SFFV-driven expression of TetR and eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
  TITLE     Development of a Multipurpose GATEWAY-Based Lentiviral 
            Tetracycline-Regulated Conditional RNAi System (GLTR).
  JOURNAL   PLoS One. 2014 May 19;9(5):e97764. doi: 
            10.1371/journal.pone.0097764. eCollection 2014.
  PUBMED    24841113
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-19"; volume: "9(5):e97764. doi"; pages: " 
            10.1371/journal.pone.0097764. eCollection 2014"
COMMENT     Sequence Label: pGLTR-X-GFP
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(84..208)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        310..711
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
       61 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
      121 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      181 ctcgttcagc tttcttgtac aaagtggttg ataattcctg cagccccgat aaaataaaag
      241 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
      301 ctagctgcag taacgccatt ttgcaaggca tggaaaaata ccaaaccaag aatagagaag
      361 ttcagatcaa gggcgggtac atgaaaatag ctaacgttgg gccaaacagg atatctgcgg
      421 tgagcagttt cggccccggc ccggggccaa gaacagatgg tcaccgcagt ttcggccccg
      481 gcccgaggcc aagaacagat ggtccccaga tatggcccaa ccctcagcag tttcttaaga
      541 cccatcagat gtttccaggc tcccccaagg acctgaaatg accctgcgcc ttatttgaat
      601 taaccaatca gcctgcttct cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa
      661 agagctcaca acccctcact cggcgcgcca gtcctccgac agactgagtc g
//
