LOCUS       sequence-96576-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PTac GFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_34
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(121..126,127..142,143..149)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    155..174
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_RNA        190..239
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     primer_bind     complement(312..340)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
       61 cgcgccgctt cgtcaggcca catagctttc ttgttctgat cggaacgatc gttggctgtg
      121 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcgctcac aattctatgg
      181 actatgtttc tgtcaccgga tgtgctttcc ggtctgatga gtccgtgagg acgaaacagc
      241 ctctacaaat aattttgttt aatcacacag gaaagtacta gatgagcaaa ggagaagaac
      301 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      361 tttctgtccg tggagagggt gaaggtgatg ctacaaacgg aaaactcacc cttaaattta
      421 tttgcactac tggaaaacta cctgttccgt ggccaacact tgtcactact ctgacctatg
      481 gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt ttcaagagtg
      541 ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac gggacctaca
      601 agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc gagttaaagg
      661 gtattgattt taaagaagat ggaaacattc ttggacacaa actcgagtac aactttaact
      721 cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa
      781 ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa caaaatactc
      841 caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca caatctgtcc
      901 tt
//
