LOCUS       sequence-96584-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express wild-type form of Ran-mRFP1 in mammalian cells (CMV 
            promoter). Also suitable for in vitro transcription (T7 promoter) 
            for injecting mRNA into oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-RanWT-mRFP1-polyA
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..23
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gcccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc
       61 gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgcgcacct tgaagcgcat
      121 gaactccttg atgacgtcct cggaggaggc cattggatcc aggtcatcat cctcatctgg
      181 gagagcagtc gtctgagcaa cctctaaatc atgctcgtac tgtgctgcca aagctgggtc
      241 catgaccacc tcaggtgggg caagagcagg catggcaaca aactccaagt taggatctcc
      301 aatgagcttt ctggcaagcc agaggaaagg cttttcaaag ttgtagttac ttttggcaga
      361 aatgtcatag tactgaagat tcttcttccg gtggaagaca atagattttg ccttcacttt
      421 cctgtcttta atatccactt tgttgccaca caatacaatg gggatgtttt cacacactcg
      481 taccagatct ctatgccagt taggtacatt cttgtaagta actcttgagg ttacatcaaa
      541 cattataatg gcacactggg cttggatgta gtagccatcg cgcaggcccc cgaacttctc
      601 ctggccggcc gtgtcccaca cgttgaactt gatgggtcct ctgttggtat ggaagacgag
      661 cgggtgcacc tccacgccca gggtggctac atacttcttc tcaaactcgc ccgtcaagtg
      721 gcgcttcacg aa
//
