LOCUS       sequence-96604-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein,
            flanked by ITRs for AAV packaging. Combine with N-Terminal Split 
            Cas9 Gyra Intein for full length SpCas9 production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
  TITLE     Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human 
            cells using compact expression cassettes.
  JOURNAL   Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
  PUBMED    26126518
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-07-1"; volume: "5:10777. doi"; pages: " 
            10.1038/srep10777"
COMMENT     Sequence Label: C-Terminal Split Cas9 with GyrA intein
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          568..795
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
ORIGIN
        1 cgatgttctc gggcttgtgc cggcccatca ctttcacgag ctcgtccacc accttcactg
       61 tctgcaggat gcccttctta atggcggggc tgccggccag attggcaatg tgctcgtgca
      121 ggctatcgcc ctggccggac acctgggctt tctggatgtc ctctttaaag gtcaggctgt
      181 cgtcgtggat cagctgcatg aagtttctgt tggcgaagcc gtcggacttc aggaaatcca
      241 ggattgtctt gccggactgc ttgtcccgga tgccgttgat cagcttccgg ctcagcctgc
      301 cccagccggt attgtgacta acgaagccat tggtaataaa agcatggtcg gctgtgtcca
      361 ccctaagaga atacacaggc tgtacgcctg catcggtcac agaggcgacc ttagcataat
      421 aaaacctccc gtcggtcagc tcgtctgcga ttgcctgagc gtctggatcc ctgtggtggg
      481 cttccaggaa tcgaaccagc cctgggacgc cgacagtata tgtagttggg gcaaattccg
      541 gttttcccat ggtggcaccg gtccaacctg aaaaaaagtg atttcaggca ggtgctccag
      601 gtaattaaac attaataccc caccaaccaa ccatccctta aacccttacc tcttgctcag
      661 ctaattacag cccggaggag aagggccgtc ccgcccgctc acctgtggga gtaacgcggt
      721 cagtcagagc cggggcgggc ggcgcgaggc ggcggcggag cggggcacgg ggcgaaggca
      781 gcgcgcagcg actcccgccc gccgcgcgct tcgcttttta tagggccgcc gccgccgccg
      841 cctcgccata aaaggaaact ttcggagcgc gccgctctga ttggctgccg ccgcacct
//
